Abstract
During the past decade, Chromosome Conformation Capture (3C/Hi-C)-based methods have been used to probe the 3D structure and organization of bacterial genomes, revealing fundamental aspects of chromosome dynamics. However, the current protocols are expensive, inefficient, and limited in their resolution. Here we present a simple, cost-effective Hi-C approach that is readily applicable to a range of Gram-positive and Gram-negative bacteria.
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References
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Acknowledgments
We thank Romain Koszul and the members of the RSG lab for insightful discussions regarding the development of this protocol. This research was supported by funding to Romain Koszul from the Agence Nationale pour la Recherche (HiResBac ANR-15-CE11-0023-03) and from the European Research Council under the Horizon 2020 program (ERC grant agreement: 771813).
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Thierry, A., Cockram, C. (2022). Generating High-Resolution Hi-C Contact Maps of Bacteria. In: Bicciato, S., Ferrari, F. (eds) Hi-C Data Analysis. Methods in Molecular Biology, vol 2301. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1390-0_9
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DOI: https://doi.org/10.1007/978-1-0716-1390-0_9
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