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Myofibroblasts pp 115–122Cite as

Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2299))

Abstract

Synthesis, deposition, and cross-linking of collagen are hallmarks of fibroblast to myofibroblast differentiation. Standard methods for determining collagen from tissue samples are not directly applicable to cell culture conditions, where the overall synthesis and deposition of collagen is clearly unfavorable, mainly due to quantity limitations and dilution of required extracellular remodeling factors. In this chapter, we describe the methods we have established to analyze collagen production and deposition into the extracellular matrix by cultured myo/fibroblasts, as well as to determine lysyl oxidase (LOX) activity in cell supernatants as an index of the capacity of the cell to cross-link collagen in vitro.

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Authors and Affiliations

  1. Centro de Biología Molecular “Severo Ochoa” Consejo Superior de Investigaciones Científicas (C.S.I.C.), Universidad Autónoma de Madrid, Madrid, Spain

    Tamara Rosell-García & Fernando Rodriguez-Pascual

Authors
  1. Tamara Rosell-García
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  2. Fernando Rodriguez-Pascual
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Corresponding author

Correspondence to Fernando Rodriguez-Pascual .

Editor information

Editors and Affiliations

  1. Laboratory of Tissue Repair and Regeneration, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada

    Boris Hinz

  2. Matrix and Mechanobiology Program, Massachusetts General Hospital, Charlestown, MA, USA

    David Lagares

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Rosell-García, T., Rodriguez-Pascual, F. (2021). Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro. In: Hinz, B., Lagares, D. (eds) Myofibroblasts. Methods in Molecular Biology, vol 2299. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1382-5_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1382-5_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1381-8

  • Online ISBN: 978-1-0716-1382-5

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