Abstract
The p53 tumor suppressor has a central role in many key cellular processes including the DNA damage response, aging, stem cell differentiation, and fertility. p53 undergoes extensive regulatory post-translational modification through events such as phosphorylation, acetylation, methylation, and ubiquitylation. Here, we describe western blotting-based methodology for the detection and relative quantification of individual phosphorylation events in p53. While we focus on well-established N-terminal modifications for the purpose of illustration, this approach can be used to investigate other post-translational modifications of the protein, drawing upon a broad range of commercially available modification-specific antibodies.
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Acknowledgments
This work was supported by a studentship from Breast Cancer Now (UK), grant number: 2013NovPhD134.
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Barber, A.E., Meek, D.W. (2021). Detection of Post-translationally Modified p53 by Western Blotting. In: Manfredi, J.J. (eds) Cell Cycle Checkpoints. Methods in Molecular Biology, vol 2267. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1217-0_2
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DOI: https://doi.org/10.1007/978-1-0716-1217-0_2
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1216-3
Online ISBN: 978-1-0716-1217-0
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