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Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures

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Melanoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2265))

Abstract

The presence of tertiary lymphoid structures (TLS) is correlated with prolonged patient survival in a variety of solid cancers, including melanoma. However, few methods have been described that could enable a more comprehensive understanding of the organization and functionality of TLS in solid cancers. In this chapter, we describe multiplex immunohistochemistry and microscopy approaches for identifying, characterizing, and quantifying TLS and intra-tumoral immune infiltrates in melanoma. The described methods are not limited to melanoma alone and could be used to evaluate tertiary lymphoid structures in a wide variety of human cancers.

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Acknowledgments

Funding: This work was supported by the University of Virginia Cancer Center (NIH P30 CA044579, Biorepository and Tissue Research Facility, Molecular and Immunologic Translational Science Core).

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Authors and Affiliations

  1. Department of Surgery, University of Virginia, Charlottesville, VA, USA

    Ileana S. Mauldin, Adela Mahmutovic, Samuel J. Young & Craig L. Slingluff Jr.

Authors
  1. Ileana S. Mauldin
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  2. Adela Mahmutovic
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  3. Samuel J. Young
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  4. Craig L. Slingluff Jr.
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Corresponding author

Correspondence to Ileana S. Mauldin .

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Editors and Affiliations

  1. Department of Biology, Hampden-Sydney College, Hampden-Sydney, VA, USA

    Kristian M. Hargadon

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Mauldin, I.S., Mahmutovic, A., Young, S.J., Slingluff, C.L. (2021). Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures. In: Hargadon, K.M. (eds) Melanoma. Methods in Molecular Biology, vol 2265. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_40

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  • DOI: https://doi.org/10.1007/978-1-0716-1205-7_40

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1204-0

  • Online ISBN: 978-1-0716-1205-7

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