Abstract
Immuno-PCR (IPCR) is a sensitive antigen detection by means of specific antibody-DNA conjugates. To ensure the successful conjugation of a protein (an antibody) with a reporter DNA, immuno-PCR method based on cDNA display (cD-IPCR) has been introduced. The cDNA display molecule is a 1:1 covalent complex of a polypeptide and its encoding cDNA at the single molecule level, which is directly used for antigen detection and subsequent qPCR. This method can be applied to detect various antigens in biological samples, if sequences of their single-domain antibodies (VHHs) or peptide aptamers are known.
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Acknowledgments
We are grateful to Dr. Takuya Terai, Hiroki Anzai, and Takeru Suzuki for helpful discussion. This work was, in part, supported by JSPS KAKENHI (15H05723 and 17K19471 to N.N.).
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Jayathilake, C., Nemoto, N. (2021). cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection. In: Posch, A. (eds) Proteomic Profiling. Methods in Molecular Biology, vol 2261. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1186-9_19
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DOI: https://doi.org/10.1007/978-1-0716-1186-9_19
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