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Inhibition of Chondroitin Sulfate Proteoglycans by APRIL

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The TNF Superfamily

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2248))

Abstract

Chondroitin sulfate proteoglycans (CSPGs) are major constituents of the extracellular matrix and well-established obstacles to regeneration in the central nervous system. As such, they are promising targets for therapy in neurological pathologies where repair is needed, such as spinal cord injuries, and multiple sclerosis. Since CSPGs mediate their inhibitory functions by interacting with signaling protein partners through their variably sulfated chondroitin sulfate glycosaminoglycan (CS-GAG) chains, blocking these epitopes presents a path to promoting repair. A member of the tumor necrosis factor (TNF) superfamily, a proliferation-inducing ligand (APRIL) has been shown to bind to CSPGs. Here we describe in vitro methods to evaluate APRIL’s ability to block CSPGs from interacting with their partner proteins and promote neuronal growth.

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Acknowledgments

We thank Pascal Schneider (University of Lausanne) for sharing his protocol for mutation/demutation of plasmids, and Leticia Peris (Grenoble Institute of Neurosciences) for sharing her expertise for the culture of neurons.

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Authors and Affiliations

  1. Team Analytic Immunology of Chronic Diseases, UGA/INSERM U1209/CNRS UMR 5309, Institute for Advanced Biosciences, La Tronche, France

    Mashal Claude Ahmed & Bertrand Huard

Authors
  1. Mashal Claude Ahmed
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  2. Bertrand Huard
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Editor information

Editors and Affiliations

  1. INSERM, Sorbonne Université, Paris, France

    Jagadeesh Bayry

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Ahmed, M.C., Huard, B. (2021). Inhibition of Chondroitin Sulfate Proteoglycans by APRIL. In: Bayry, J. (eds) The TNF Superfamily. Methods in Molecular Biology, vol 2248. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1130-2_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1130-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1129-6

  • Online ISBN: 978-1-0716-1130-2

  • eBook Packages: Springer Protocols

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