Abstract
Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples.
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Acknowledgements
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UIDB/50011/2020 & UIDP/50011/2020) and LAQV-Associate Laboratory for Green Chemistry (Ref. FCT UIDB/50006/2020), financed by national funds through the FCT/MCTES, and when appropriate cofinanced by FEDER under the PT2020 Partnership Agreement. Paulo C. Lemos acknowledges the support of FCT/MCTES for contract IF/01054/2014/CP1224/CT0005. Diogo Queirós and Joana Pereira thank FCT/MCTES for their PhD grants, SFRH/BD/87758/2012 and SFRH/BD/130003/2017, respectively.
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Di Pippo, F., Queirós, D., Pereira, J., Lemos, P.C., Serafim, L.S., Rossetti, S. (2021). FISH in Suspension or in Adherent Cells. In: Azevedo, N.F., Almeida, C. (eds) Fluorescence In-Situ Hybridization (FISH) for Microbial Cells. Methods in Molecular Biology, vol 2246. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1115-9_4
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DOI: https://doi.org/10.1007/978-1-0716-1115-9_4
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