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Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions

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Human Cytomegaloviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2244))

Abstract

Human cytomegalovirus (HCMV) entry into host cells is a complex process involving interactions between an array of viral glycoproteins with multiple host cell surface receptors. A significant amount of research has been devoted toward identifying these glycoprotein and cellular receptor interactions as the broad cellular tropism of HCMV suggests a highly regulated yet adaptable process that controls viral binding and penetration. However, deciphering the initial binding and cellular receptor activation events by viral glycoproteins remains challenging. The relatively low abundance of receptors and/or interactions with glycoproteins during viral entry, the hydrophobicity of membrane receptors, and the rapid degradation and recycling of activated receptors have complicated the analysis of HCMV entry and the cellular signaling pathways initiated by HCMV engagement to the host membrane. Here, we describe the different methodologies used in our laboratory and others to analyze the interactions between HCMV glycoproteins and host cellular receptors during the entry stage of the viral life cycle.

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Acknowledgments

This work was supported by grants from the Carol M. Baldwin Breast Cancer Research Fund to G.C. Chan, National Institute of Allergy and Infectious Disease (R01AI141460) to G.C. Chan, and National Heart, Lung, and Blood Institute (R01HL139824) to G.C. Chan.

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Authors and Affiliations

  1. Department of Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, NY, USA

    Jamil Mahmud & Gary C. Chan

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  1. Jamil Mahmud
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  2. Gary C. Chan
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Corresponding author

Correspondence to Gary C. Chan .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Louisiana State University Health Sciences Center—Shreveport, Shreveport, LA, USA

    Andrew D. Yurochko

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Mahmud, J., Chan, G.C. (2021). Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions. In: Yurochko, A.D. (eds) Human Cytomegaloviruses. Methods in Molecular Biology, vol 2244. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1111-1_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1111-1_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1110-4

  • Online ISBN: 978-1-0716-1111-1

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