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Antibody Arrays pp 55–67Cite as

Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2237))

Abstract

The coupling of surface plasmon resonance imaging (SPRi) with mass spectrometry (MS) offers a very promising multidimensional analysis. This system takes advantage of the two well-established techniques: SPR, which allows for the analysis of biomolecular interactions through the determination of kinetic and thermodynamic constants, and MS, which can characterize biological structures from mass measurements and fragmentation experiments. Here, a protocol for the coupling of SPRi with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described using a biochip grafted by antibodies in an array format. Interaction between β-lactoglobulin antibodies and the protein antigen is detected and analyzed by SPRi. Then, the arrayed biochip which fitted a commercially MALDI target was inserted in a MALDI source, and mass spectra were recorded directly from the biochip surface from each antibody spot, showing protein ions attributed to the corresponding specific protein antigens.

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Acknowledgments

Authors are indebted to Dr. D. Lebeau for access to the MALDI-TOF/TOF MS instrument (Den-Service d’Etude du Comportement des Radionucléides, CEA, Paris-Saclay University, France); A. H. acknowledges fellowships from Université d’Evry—Paris Saclay and Région Ile-de France (Dim Analytics).

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Authors and Affiliations

  1. Université Paris-Saclay, Univ Evry, CNRS, LAMBE, Evry-Courcouronnes, Paris, France

    Anastasiia Halushkina, William Buchmann, Nathalie Jarroux & Régis Daniel

  2. CY Cergy Paris Université, CNRS, LAMBE, Cergy, Paris, France

    Anastasiia Halushkina, William Buchmann, Nathalie Jarroux & Régis Daniel

Authors
  1. Anastasiia Halushkina
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  2. William Buchmann
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  3. Nathalie Jarroux
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  4. Régis Daniel
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Corresponding author

Correspondence to Régis Daniel .

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Editors and Affiliations

  1. RayBiotech, Peachtree Corners, GA, USA

    Kelly C. Whittaker

  2. RayBiotech Life, Peachtree Corners, GA, USA

    Ruo-Pan Huang

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Halushkina, A., Buchmann, W., Jarroux, N., Daniel, R. (2021). Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen. In: Whittaker, K.C., Huang, RP. (eds) Antibody Arrays. Methods in Molecular Biology, vol 2237. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1064-0_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1064-0_5

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1063-3

  • Online ISBN: 978-1-0716-1064-0

  • eBook Packages: Springer Protocols

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