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CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei

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Trichoderma reesei

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2234))

Abstract

In this protocol, we describe the establishment of a CRISPR/Cas9 system in Trichoderma reesei by generating a specific, codon-optimized Cas9-expressing strain and by in vitro transcription of a gRNA. This system induces mutagenesis or introduces a gene in a targeted way based on PEG-mediated protoplast transformation. Up to three targets, multiplexed genome editing can be obtained in one transformation.

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Author information

Authors and Affiliations

  1. CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

    Gen Zou & Zhihua Zhou

Authors
  1. Gen Zou
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  2. Zhihua Zhou
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Corresponding author

Correspondence to Zhihua Zhou .

Editor information

Editors and Affiliations

  1. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Astrid R. Mach-Aigner

  2. Christian Doppler Laboratory for Optimized Expression of Carbohydrate-Active Enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Roland Martzy

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Zou, G., Zhou, Z. (2021). CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei. In: Mach-Aigner, A.R., Martzy, R. (eds) Trichoderma reesei. Methods in Molecular Biology, vol 2234. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1048-0_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1048-0_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1047-3

  • Online ISBN: 978-1-0716-1048-0

  • eBook Packages: Springer Protocols

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