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Epigenetic Reprogramming During Mouse Embryogenesis pp 143–155Cite as

Whole-Mount Immunofluorescence Staining of Early Mouse Embryos

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2214))

Abstract

Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the sample processing, protein detection using antibodies and confocal imaging of isolated preimplantation to early postimplantation mouse embryos up to Embryonic day 8.0 (E8.0).

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Author information

Authors and Affiliations

  1. Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK

    Frederick C. K. Wong

Authors
  1. Frederick C. K. Wong
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Correspondence to Frederick C. K. Wong .

Editor information

Editors and Affiliations

  1. Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Paris, France

    Katia Ancelin

  2. Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Institut de Ge´ne´tique Mole´culaire de Montpellier, Univ Montpellier, CNRS Montpellier, France, Paris, France

    Maud Borensztein

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Wong, F.C.K. (2021). Whole-Mount Immunofluorescence Staining of Early Mouse Embryos. In: Ancelin, K., Borensztein, M. (eds) Epigenetic Reprogramming During Mouse Embryogenesis. Methods in Molecular Biology, vol 2214. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0958-3_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0958-3_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0957-6

  • Online ISBN: 978-1-0716-0958-3

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