Abstract
Adhesive pili (or fimbriae) in bacteria are classified into five types, among which type V pili have been most recently described. Type V pili differ from other pili types with respect to transport mechanism, structure, and pilin synthesis. Genes of type V pili are restricted to the phylum Bacteroidetes. Protein subunits that compose type V pili are transported to the cell surface as lipoprotein precursors and then polymerized into a pilus through a strand-exchange mechanism, which is demonstrated by several experiments, including palmitic acid labeling and Cys-Cys cross-linking analysis. Here, we describe the use of these methods to analyze type V pili.
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We thank Editage (www.editage.com) and Matthews MM (Okinawa Institute of Science and Technology Graduate University) for English language editing.
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Shoji, M., Shibata, S., Naito, M., Nakayama, K. (2021). Transport and Polymerization of Porphyromonas gingivalis Type V Pili. In: Nagano, K., Hasegawa, Y. (eds) Periodontal Pathogens. Methods in Molecular Biology, vol 2210. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0939-2_7
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DOI: https://doi.org/10.1007/978-1-0716-0939-2_7
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