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Structural Genomics pp 67–94Cite as

Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2199))

Abstract

This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA–DNA processing, and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 mL of insect cell culture and describe the procedures involved in producing protein from liter-scale cultures.

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Acknowledgments

We would like to thank all the SGC scientists (past and present) who contributed toward the development of the method. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada, Innovative Medicines Initiative (EU/EFPIA), Janssen, Merck KGaA, MSD, Novartis Pharma AG, Ontario Ministry of Economic Development and Innovation, Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome. The BacMam vector backbone (pHTBV1.1 ) was kindly provided by Professor Frederick Boyce (Massachusetts General Hospital, Cambridge, MA). 

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Authors and Affiliations

  1. Astex Pharmaceuticals, Cambridge, UK

    Pravin Mahajan

  2. Diamond Light Source Ltd., Didcot, Oxfordshire, UK

    Claire Strain-Damerell

  3. Structural Genomics Consortium, University of Oxford, Oxford, UK

    Shubhashish Mukhopadhyay, Alejandra Fernandez-Cid, Opher Gileadi & Nicola A. Burgess-Brown

Authors
  1. Pravin Mahajan
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  2. Claire Strain-Damerell
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  3. Shubhashish Mukhopadhyay
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  4. Alejandra Fernandez-Cid
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  5. Opher Gileadi
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  6. Nicola A. Burgess-Brown
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Corresponding author

Correspondence to Nicola A. Burgess-Brown .

Editor information

Editors and Affiliations

  1. Department of Applied Biology and Chemical Technology and the State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hung Hom, Hong Kong

    Yu Wai Chen

  2. BMI Biotechnology Appraisals & Investments Limited, Wan Chai, Hong Kong

    Chin-Pang Bennu Yiu

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Mahajan, P., Strain-Damerell, C., Mukhopadhyay, S., Fernandez-Cid, A., Gileadi, O., Burgess-Brown, N.A. (2021). Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells. In: Chen, Y.W., Yiu, CP.B. (eds) Structural Genomics. Methods in Molecular Biology, vol 2199. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0892-0_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0892-0_5

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0891-3

  • Online ISBN: 978-1-0716-0892-0

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