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Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection

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Testicular Germ Cell Tumors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2195))

Abstract

Testicular germ cell tumors are among the most common malignancies seen in children and young adults. Genomic studies have identified characteristic molecular profiles in testicular cancer, which are associated with histologic subtypes and may predict clinical behavior including treatment responses. Emerging molecular technologies analyzing tumor genomics, transcriptomics, and proteomics may now guide precision management of testicular tumors. Laser-assisted microdissection methods such as laser capture microdissection efficiently isolate selected tumor cells from routine pathology specimens, avoiding contamination from nontarget cell populations. Laser capture microdissection in combination with next generation sequencing makes precise high throughput genetic evaluation effective and efficient. The use of laser capture microdissection (LCM) for molecular testing may translate into great benefits for the clinical management of patients with testicular cancers. This review discusses application protocols for laser-assisted microdissection to investigate testicular germ cell tumors.

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Funding

This work was supported by the Slovak Research and Development Agency under contract No. APVV-15-0086 and Scientific Grant Agency under contract number VEGA 1/0043/18 for Michal Chovanec.

Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA

    Liang Cheng, Steven A. Mann, Mingsheng Wang, Darrell D. Davidson & Shaobo Zhang

  2. Department of Urology, Indiana University School of Medicine, Indianapolis, IN, USA

    Liang Cheng

  3. Department of Pathology and Surgery, Faculty of Medicine, University of Cordoba, Cordoba, Spain

    Antonio Lopez-Beltran

  4. Pathology Service, Champalimaud Clinical Center, Lisbon, Portugal

    Antonio Lopez-Beltran

  5. 2nd Department of Oncology, Faculty of Medicine, Comenius University and National Cancer Institute, Bratislava, Slovakia

    Michal Chovanec

  6. Division of Hematology and Oncology, Indiana University Simon Cancer Center, Indianapolis, IN, USA

    Michal Chovanec

  7. Oncology Unit, Macerata Hospital, Macerata, Italy

    Matteo Santoni

  8. Section of Pathological Anatomy, Polytechnic University of the Marche Region, School of Medicine, United Hospitals, Ancona, Italy

    Alessia Cimadamore & Rodolfo Montironi

  9. Department of Medicine, Division of Hematology and Oncology, Indiana University Simon Cancer Center, Indianapolis, IN, USA

    Costantine Albany & Nabil Adra

Authors
  1. Liang Cheng
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  2. Steven A. Mann
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  3. Antonio Lopez-Beltran
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  4. Michal Chovanec
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  6. Mingsheng Wang
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  7. Costantine Albany
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  8. Nabil Adra
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  9. Darrell D. Davidson
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  10. Alessia Cimadamore
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  11. Rodolfo Montironi
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  12. Shaobo Zhang
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Editor information

Editors and Affiliations

  1. Department of Urology, UT Southwestern Medical Center, Dallas, TX, USA

    Aditya Bagrodia

  2. Cancer and Blood Disease Institute, Children’s Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

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Cheng, L. et al. (2021). Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection. In: Bagrodia, A., Amatruda, J.F. (eds) Testicular Germ Cell Tumors. Methods in Molecular Biology, vol 2195. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0860-9_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0860-9_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0859-3

  • Online ISBN: 978-1-0716-0860-9

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