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Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men

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Testicular Germ Cell Tumors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2195))

Abstract

Germ cell tumors (GCT) in men comprise of tumor subtypes with distinct histomorphologies, genetic and genomic alterations, and clinical behavior. Immunohistochemical (IHC) markers, including many newly described nuclear transcription factors, are often applied in challenging cases to arrive at a correct diagnosis and classification, and to establish germ cell origin for metastatic tumors. However, there is no established role for IHC markers in prognosis and therapy response prediction in GCTs. This chapter briefly reviews the clinical utility of IHC in diagnosis and classification of GCTs, including technical aspects of performing IHC and clinical applications of commonly used IHC markers in the workup of common and clinically relevant diagnostic scenarios.

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Abbreviations

β-HCG :

Beta subunit of human chorionic gonadotropin

AFP :

Alpha-fetoprotein

EC :

Embryonal carcinoma

GCT:

Germ cell tumor

GPC3:

Glypican 3

IHC:

Immunohistochemistry

PLAP:

Placental alkaline phosphatase

YST:

Yolk sac tumor

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Author information

Authors and Affiliations

  1. Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Franto Francis & Ming Zhou

  2. Tufts Medical Center and Tufts School of Medicine, Boston, MA, USA

    Ming Zhou

Authors
  1. Franto Francis
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  2. Ming Zhou
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Corresponding author

Correspondence to Ming Zhou .

Editor information

Editors and Affiliations

  1. Department of Urology, UT Southwestern Medical Center, Dallas, TX, USA

    Aditya Bagrodia

  2. Cancer and Blood Disease Institute, Children’s Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

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Francis, F., Zhou, M. (2021). Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men. In: Bagrodia, A., Amatruda, J.F. (eds) Testicular Germ Cell Tumors. Methods in Molecular Biology, vol 2195. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0860-9_2

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  • DOI: https://doi.org/10.1007/978-1-0716-0860-9_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0859-3

  • Online ISBN: 978-1-0716-0860-9

  • eBook Packages: Springer Protocols

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