Abstract
In just over 10 years, the use of optogenetic technologies in neuroscience has become widespread, having today a tremendous impact on our understanding of brain function. An extensive number of studies have implemented a variety of tools allowing for the manipulation of neurons with light, including light-activated ion channels or G protein-coupled receptors, among other innovations. In this context, the proper calibration of photostimulation in vivo remains crucial to dissect brain circuitry or investigate the effect of neuronal activity on specific subpopulations of neurons and glia. Depending on the scientific question, the design of specific stimulation protocols must consider from the choice of the animal model to the light stimulation pattern to be delivered. In this chapter, we describe a detailed framework to investigate neuron-glia interactions in both mouse pups and adults using an optogenetic approach.
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Acknowledgments
This work was supported by grants from Fondation pour la Recherche Médicale (FRM; “Equipe FRM DEQ20150331681”), Fondation pour l’aide à la recherche sur la sclérose en plaques (ARSEP), a subaward agreement from the University of Connecticut with funds provided by Grant No. RG-1612-26501 from the National Multiple Sclerosis Society (NMSS) to M.C.A. and from ECOS-Sud (Project No. ECOS180013) to M.C.A. and F.C.O. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of UConn or NMSS. F.C.O. was recipient of a FRM post-doctoral fellowship and is supported by the Chilean National Fund for Scientific and Technological Development (FONDECYT, INICIACION #11160616). F.C.O. and C.H. were recipients of ARSEP post-doctoral fellowships. B.M.S. was supported by a PhD fellowship from the French Ministry of Research (ED BioSPC), and D.O. was supported by a INSPIRE PhD fellowship from Marie Skłodowska-Curie Action (European Union’s Horizon 2020) and Université de Paris (grant agreement: 665850; ED BioSPC).
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Habermacher, C., Manot-Saillet, B., Ortolani, D., Ortiz, F.C., Angulo, M.C. (2021). Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults. In: Dempski, R. (eds) Channelrhodopsin. Methods in Molecular Biology, vol 2191. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0830-2_9
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DOI: https://doi.org/10.1007/978-1-0716-0830-2_9
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