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Analysis of Immune-Tumor Cell Interactions Using a 3D Co-culture Model

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Immunometabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2184))

Abstract

Three-dimensional (3D) cultures are better able to reflect the tumor microenvironment than two-dimensional (2D) monolayer cultures by facilitating cell-cell interactions in the appropriate spatial dimensions. Here I describe the isolation and co-culture of immune cells with tumor cell lines in a three-dimensional system facilitated by a basement membrane extract. This allows for further downstream applications to analyze interactions between these cell types.

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References

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Authors and Affiliations

  1. School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

    Tanya N. Augustine

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  1. Tanya N. Augustine
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Corresponding author

Correspondence to Tanya N. Augustine .

Editor information

Editors and Affiliations

  1. Faculty of Health Sciences, Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada

    Suresh Mishra

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Augustine, T.N. (2020). Analysis of Immune-Tumor Cell Interactions Using a 3D Co-culture Model. In: Mishra, S. (eds) Immunometabolism. Methods in Molecular Biology, vol 2184. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0802-9_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0802-9_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0801-2

  • Online ISBN: 978-1-0716-0802-9

  • eBook Packages: Springer Protocols

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