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CRISPR-Cas9-Based Genome Editing of Banana

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CRISPR-Cas Methods

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Genome editing is an emerging powerful new breeding tool, which can be applied for genetic improvement of banana for important agronomic traits such as resistance to biotic stresses, adaptation to climate change, and high yielding. Banana is an important staple food and cash crop, feeding millions of people in tropical and subtropical countries. Recently, CRISPR-Cas9-based genome editing system has been established for banana in a few laboratories. Here, we describe the procedures for generation of genome-edited events of banana, detection of targeted and potential off-target mutations, and phenotyping for important traits such as disease resistance. This chapter will provide readers strategy for applying CRISPR-Cas9-based genome editing for improvement of banana.

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Author information

Authors and Affiliations

  1. International Institute of Tropical Agriculture (IITA), Nairobi, Kenya

    Leena Tripathi, Valentine Otang Ntui & Jaindra Nath Tripathi

Authors
  1. Leena Tripathi
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  2. Valentine Otang Ntui
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  3. Jaindra Nath Tripathi
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Corresponding author

Correspondence to Leena Tripathi .

Editor information

Editors and Affiliations

  1. Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

    M. Tofazzal Islam

  2. Department of Aquatic and Crop Resource Development, National Research Council of Canada, SASKATOON, SK, Canada

    Pankaj K. Bhowmik

  3. Crop Improvement Division, ICAR-National Rice Research Institute, Cuttack, Odisha, India

    Kutubuddin A. Molla

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Tripathi, L., Ntui, V.O., Tripathi, J.N. (2020). CRISPR-Cas9-Based Genome Editing of Banana. In: Islam, M.T., Bhowmik, P.K., Molla, K.A. (eds) CRISPR-Cas Methods . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0616-2_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0616-2_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0615-5

  • Online ISBN: 978-1-0716-0616-2

  • eBook Packages: Springer Protocols

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