Abstract
The discovery and subsequent repurposing of the CRISPR (clustered regularly interspaced short palindromic repeats) and Cas proteins (CRISPR-associated proteins) has revolutionized our ability to manipulate DNA and RNA sequences in vitro, ex vivo, and in vivo. In this introductory chapter, we present a brief overview of basics of CRISPR-Cas-mediated genome editing and different orthologues and engineered versions of Cas protein with altered specificity and expanded targetability. More importantly, we comprehensively portray the advances made by developing diverse CRISPR-Cas-based genome modification tools and their application in basic biology, agriculture, and medicine.
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Molla, K.A., Karmakar, S., Islam, M.T. (2020). Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine. In: Islam, M.T., Bhowmik, P.K., Molla, K.A. (eds) CRISPR-Cas Methods . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0616-2_1
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DOI: https://doi.org/10.1007/978-1-0716-0616-2_1
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