Abstract
In vivo calcium imaging in zebrafish provides the ability to investigate calcium dynamics within neurons. Utilizing genetically encoded calcium sensors it is possible to monitor calcium signals within a single axon during axon injury and degeneration with high temporal and spatial resolution. Here we will describe in vivo, time-lapse confocal imaging methods of calcium imaging. Imaging of calcium dynamics with genetically encoded calcium sensors (GECS) within living axons can serve as a method to assess axonal physiology and effects of pharmacologic and genetic manipulation, as well as characterize responses to different injury models.
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Video 1
Time-lapse movie of axonal fragmentation and calcium dynamics following axotomy. Sensory axon is labeled with RPF and GCaMP-HS. Imaged at 1 min intervals starting at 15 min postaxotomy (AVI 3968 kb) (AVI 3968 kb)
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Vargas, M.E. (2020). In Vivo Calcium Imaging During Axon Degeneration in Zebrafish. In: Babetto, E. (eds) Axon Degeneration. Methods in Molecular Biology, vol 2143. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0585-1_19
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DOI: https://doi.org/10.1007/978-1-0716-0585-1_19
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