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Biolistic DNA Delivery and Its Applications in Sorghum bicolor

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Biolistic DNA Delivery in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2124))

Abstract

Biolistic DNA delivery has been considered a universal tool for genetic manipulation to transfer exotic genes to cells or tissues due to its simplicity, versatility, and high efficiency. It has been a preferred method for investigating plant gene function in most monocot crops. The first transgenic sorghum plants were successfully regenerated through biolistic DNA delivery in 1993, with a relatively low transformation efficiency of 0.3%. Since then, tremendous progress has been made in recent years where the highest transformation efficiency was reported at 46.6%. Overall, the successful biolistic DNA delivery system is credited to three fundamental cornerstones: robust tissue culture system, effective gene expression in sorghum, and optimal parameters of DNA delivery. In this chapter, the history, application, and current development of biolistic DNA delivery in sorghum are reviewed, and the prospect of sorghum genetic engineering is discussed.

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Acknowledgments

We are thankful to GRDC (Grains Research & Development Corporation) for funding the research project 9176038 (Better sorghum: larger grain with more protein).

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Authors and Affiliations

  1. Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Australia

    Guoquan Liu, Karen Massel, Basam Tabet & Ian D. Godwin

Authors
  1. Guoquan Liu
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  2. Karen Massel
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  3. Basam Tabet
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  4. Ian D. Godwin
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Corresponding author

Correspondence to Guoquan Liu .

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Editors and Affiliations

  1. Department of Plant and Environmental Sciences, School of Health Research, Pee Dee Research and Education Center, Clemson University, Florence, SC, USA

    Sachin Rustgi

  2. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, USA

    Hong Luo

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Liu, G., Massel, K., Tabet, B., Godwin, I.D. (2020). Biolistic DNA Delivery and Its Applications in Sorghum bicolor. In: Rustgi, S., Luo, H. (eds) Biolistic DNA Delivery in Plants. Methods in Molecular Biology, vol 2124. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0356-7_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0356-7_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0355-0

  • Online ISBN: 978-1-0716-0356-7

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