Abstract
Plant embryogenesis begins with fertilization and ends with the generation of the basic body plan of the future plant. Despite its importance, the dynamics of flowering plant ontogeny have long been a mystery, because the embryo develops deep in the maternal tissue. Recently, an embryonic live-cell imaging system was established in Arabidopsis thaliana by developing an in vitro ovule cultivation method and utilizing two-photon excitation microscopy (2PEM), which is suitable for deep imaging. This system enabled us to visualize intracellular dynamics during zygote polarization and monitor the cell division pattern during embryogenesis from the zygote until organ formation. In this chapter, we describe a method that allows for high-resolution imaging of cytoskeletal rearrangements in the zygote and long-term tracing of embryo patterning.
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Acknowledgments
M.U., Y.K., and D.K. are supported by the Japan Society for the Promotion of Science (Grant-in-Aid for Scientific Research on Innovative Areas [JP17H05838 for M.U.], Grant-in-Aid for Challenging Exploratory Research [JP16K14753 for M.U.], Grant-in-Aid for JSPS Research Fellow [JP18J10512 for Y.K.], Grant-in-Aid for Scientific Research [B; JP17H03697 for D.K.], and Challenging Research [Exploratory; JP 18Â K19331 for D.K.]). This work was supported by the Institute of Transformative Bio-Molecules of Nagoya University and the Japan Advanced Plant Science Network.
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Ueda, M., Kimata, Y., Kurihara, D. (2020). Live-Cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana. In: Bayer, M. (eds) Plant Embryogenesis. Methods in Molecular Biology, vol 2122. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0342-0_4
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DOI: https://doi.org/10.1007/978-1-0716-0342-0_4
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