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Pig-to-Macaque Islet Xenotransplantation

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Xenotransplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2110))

Abstract

The advancement toward a clinical application for porcine islets to cure diabetes in humans must include reproducible long-term successes in non-human primate (NHP) models. Many dedicated researchers around the world are continuing to work toward this goal. In this chapter, we describe procedures for islet isolation of pancreatic islets from adult and neonatal/fetal pigs. We further include procedures for the induction of diabetes in non-human primates and subsequent insulin therapy, islet transplantation, immunosuppression, and also the daily maintenance of xenotransplanted NHPs. The procedures that we outline in this chapter are ones that we have successfully utilized in pig-to-NHP islet transplantation models. However, where appropriate, alternative methods will also be identified.

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Acknowledgments

The authors kindly wish to recognize and thank Anna Lisa Haughwout for her splendid illustration of the primate tether system.

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Authors and Affiliations

  1. Institute of Cellular Therapeutics, Allegheny Health Network Research Institute, Allegheny Health Network, Pittsburgh, PA, USA

    Suzanne Bertera, Michael F. Knoll, Carmela A. Knoll, Massimo Trucco & Rita Bottino

  2. Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA

    David K. C. Cooper

Authors
  1. Suzanne Bertera
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  2. Michael F. Knoll
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  3. Carmela A. Knoll
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  4. David K. C. Cooper
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  5. Massimo Trucco
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  6. Rita Bottino
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Corresponding author

Correspondence to Rita Bottino .

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Editors and Affiliations

  1. Institut d’Investigació Biomèdica de Bellvitge (IDIBELL), Hospital Duran i Reynals, L’Hospitalet de LLobregat, Barcelona, Spain

    Cristina Costa

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Bertera, S., Knoll, M.F., Knoll, C.A., Cooper, D.K.C., Trucco, M., Bottino, R. (2020). Pig-to-Macaque Islet Xenotransplantation. In: Costa, C. (eds) Xenotransplantation. Methods in Molecular Biology, vol 2110. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0255-3_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0255-3_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0254-6

  • Online ISBN: 978-1-0716-0255-3

  • eBook Packages: Springer Protocols

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