Abstract
Significant effort has been devoted to the optimization of solid-phase peptide synthesis (SPPS) to maximize the process to facilitate the synthesis of a desired peptide sequence, without extensive optimization or resynthesis. Over the last 25 years, a set of synthetic protocols developed by Kent and Alewood has proven to be robust and efficient for Boc/Bzl SPPS and has been widely adopted by the research community. In this chapter, we describe a variation of manual in situ neutralization protocols for Boc-SPPS that are highly effective for the rapid synthesis of peptides with different C-terminal functionalities.
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Acknowledgments
We thank Paul Alewood and Stephen Kent for their mentorship and inspiration.
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Adhikary, R., Dawson, P.E. (2020). In Situ Neutralization Protocols for Boc-SPPS. In: Hussein, W., Skwarczynski, M., Toth, I. (eds) Peptide Synthesis. Methods in Molecular Biology, vol 2103. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0227-0_3
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DOI: https://doi.org/10.1007/978-1-0716-0227-0_3
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