Abstract
CAR-T cell immunotherapy is a promising therapeutic modality for cancer patients. The success of CAR-T cell therapy has been associated with the phenotype, activation and functional profiling of infused CAR-T cells. Therefore, immunophenotypic characterization of CAR-T cells during bioprocess is crucial for cell quality control and ultimately for improved antitumor efficacy. In this chapter, we propose a flow cytometry panel to characterize the immunophenotype of the CAR-T subsets.
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de Azevedo, J.T.C., Mizukami, A., Moço, P.D., Malmegrim, K.C.R. (2020). Immunophenotypic Analysis of CAR-T Cells. In: Swiech, K., Malmegrim, K., Picanço-Castro, V. (eds) Chimeric Antigen Receptor T Cells. Methods in Molecular Biology, vol 2086. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0146-4_14
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DOI: https://doi.org/10.1007/978-1-0716-0146-4_14
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0146-4
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