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Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor

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Bioreactors in Stem Cell Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2436))

Abstract

Mesenchymal stem cells show great potential in tissue engineering and cell-based therapies. This protocol demonstrates the use of 3D TableTrix® microcarrier tablets for large-scale manufacturing of human umbilical cord mesenchymal stem cells (hUCMSCs) in a 5-L stirred tank bioreactor. 3D TableTrix® microcarrier tablets readily disperse into tens of thousands of porous microcarriers to simplify cell seeding, and 3D FloTrix® vivaSPIN bioreactor could automate, monitor, and control the entire culture process. 3D TableTrix® microcarriers could also be fully dissolved upon adding dissolution reagent to gently harvest the expanded cells at a high recovery rate. With this protocol, more than 109 cells could be produced in a 5-L bioreactor.

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Acknowledgments

This work is financially supported by Beijing Municipal Science & Technology Commission (Z181100001818005) and R&D program (RC21-01) of Beijing CytoNiche Biotechnology Co. Ltd.

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Author notes

  1. Huanye Xu and Zhongxiao Cong contributed equally to this work.

Authors and Affiliations

  1. Beijing CytoNiche Biotechnology Co., Ltd., Beijing, China

    Huanye Xu, Zhongxiao Cong, Yuanyuan Zhang, Wei Liu & Xiaojun Yan

  2. Department of Biomedical Engineering, School of Medicine, Tsinghua-PKU Center for Life Sciences, Tsinghua University, Beijing, China

    Yanan Du

Authors
  1. Huanye Xu
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  2. Zhongxiao Cong
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  3. Yuanyuan Zhang
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  4. Wei Liu
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  5. Xiaojun Yan
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  6. Yanan Du
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Corresponding author

Correspondence to Xiaojun Yan .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Xu, H., Cong, Z., Zhang, Y., Liu, W., Yan, X., Du, Y. (2021). Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor. In: Turksen, K. (eds) Bioreactors in Stem Cell Biology. Methods in Molecular Biology, vol 2436. Humana, New York, NY. https://doi.org/10.1007/7651_2021_425

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  • DOI: https://doi.org/10.1007/7651_2021_425

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2017-5

  • Online ISBN: 978-1-0716-2018-2

  • eBook Packages: Springer Protocols

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