Abstract
In the past decades, human induced pluripotent stem cells (iPSCs) have been generated by the ectopic expression of “Yamanaka factors” in multiple somatic cells. However, the procedure to get access to donor cells is hard or invasive in most cases. Hereon, we depict a stepwise method developed in our laboratory for the generation of iPSCs from renal epithelial cells present in urine, which is noninvasive, nonintegrating, and universal. The resulting urinary iPSCs (UiPSCs) exhibit pluripotent characteristics resemble embryonic stem cells (ESCs) and thus urine may be a favorable source for generating iPSCs.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861–872
Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science (New York, NY) 318(5858):1917–1920
Shi Y, Inoue H, Wu JC, Yamanaka S (2017) Induced pluripotent stem cell technology: a decade of progress. Nat Rev Drug Discov 16(2):115–130
Huang J, Feng Q, Wang L, Zhou B (2021) Human pluripotent stem cell-derived cardiac cells: application in disease modeling, cell therapy, and drug discovery. Front Cell Dev Biol 9:655161
Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC (2008) Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol 26(11):1276–1284
Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, Hu S, Cherry AM, Robbins RC, Longaker MT, Wu JC (2009) Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci U S A 106(37):15720–15725
Sugii S, Kida Y, Kawamura T, Suzuki J, Vassena R, Yin YQ, Lutz MK, Berggren WT, Izpisúa Belmonte JC, Evans RM (2010) Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells. Proc Natl Acad Sci U S A 107(8):3558–3563
Staerk J, Dawlaty MM, Gao Q, Maetzel D, Hanna J, Sommer CA, Mostoslavsky G, Jaenisch R (2010) Reprogramming of human peripheral blood cells to induced pluripotent stem cells. Cell Stem Cell 7(1):20–24
Seki T, Yuasa S, Oda M, Egashira T, Yae K, Kusumoto D, Nakata H, Tohyama S, Hashimoto H, Kodaira M, Okada Y, Seimiya H, Fusaki N, Hasegawa M, Fukuda K (2010) Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells. Cell Stem Cell 7(1):11–14
Liu H, Ye Z, Kim Y, Sharkis S, Jang YY (2010) Generation of endoderm-derived human induced pluripotent stem cells from primary hepatocytes. Hepatology 51(5):1810–1819
Kim JB, Greber B, Araúzo-Bravo MJ, Meyer J, Park KI, Zaehres H, Schöler HR (2009) Direct reprogramming of human neural stem cells by OCT4. Nature 461(7264):649–643
Li C, Zhou J, Shi G, Ma Y, Yang Y, Gu J, Yu H, Jin S, Wei Z, Chen F, Jin Y (2009) Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells. Hum Mol Genet 18(22):4340–4349
Takenaka C, Nishishita N, Takada N, Jakt LM, Kawamata S (2010) Effective generation of iPS cells from CD34+ cord blood cells by inhibition of p53. Exp Hematol 38(2):154–162
Haase A, Olmer R, Schwanke K, Wunderlich S, Merkert S, Hess C, Zweigerdt R, Gruh I, Meyer J, Wagner S, Maier LS, Han DW, Glage S, Miller K, Fischer P, Schöler HR, Martin U (2009) Generation of induced pluripotent stem cells from human cord blood. Cell Stem Cell 5(4):434–441
Detrisac CJ, Mayfield RK, Colwell JA, Garvin AJ, Sens DA (1983) In vitro culture of cells exfoliated in the urine by patients with diabetes mellitus. J Clin Invest 71(1):170–173
Guo X, Wang L, Chen K, Song S, Wang X, Gu X, Niu C, Chu M (2020) Generation of urine-derived iPS cell line via a non-integrative method from a Barth syndrome patient with TAZ gene mutation. Stem Cell Res 47:101886
Cao Y, Xu J, Wen J, Ma X, Liu F, Li Y, Chen W, Sun L, Wu Y, Li S, Li J, Huang G (2018) Generation of a urine-derived Ips cell line from a patient with a ventricular septal defect and heart failure and the robust differentiation of these cells to cardiomyocytes via small molecules. Cell Physiol Biochem 50(2):538–551
Zhou T, Benda C, Dunzinger S, Huang Y, Ho JC, Yang J, Wang Y, Zhang Y, Zhuang Q, Li Y, Bao X, Tse HF, Grillari J, Grillari-Voglauer R, Pei D, Esteban MA (2012) Generation of human induced pluripotent stem cells from urine samples. Nat Protoc 7(12):2080–2089
Acknowledgments
This study was supported by the National Natural Science Foundation of China (82003756, 81770257 and 81970223), the National Key R&D Program of China (2017YFA0103700), the Natural Science Foundation of Jiangsu Province (BK20200880, BK20170002, and BK20201409), the Natural Science Foundation for Colleges and Universities in Jiangsu Province (17KJA310006), Jiangsu Province’s Key Discipline/Laboratory of Medicine (XK201118), National Center for International Research (2017B01012), and Introduction Project of Clinical Medicine Expert Team for Suzhou (SZYJTD201704).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Yu, M., Han, X., Ye, L., Lei, W., Hu, S. (2021). Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_419
Download citation
DOI: https://doi.org/10.1007/7651_2021_419
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2584-2
Online ISBN: 978-1-0716-2585-9
eBook Packages: Springer Protocols