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Bioreactors in Stem Cell Biology pp 205–222Cite as

Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2436))

Abstract

With the aging population, the demand for artificial small diameter vascular grafts is constantly increasing, as the availability of autologous grafts is limited due to vascular diseases. A confluent lining with endothelial cells is considered to be a cornerstone for long-term patency of artificial small diameter grafts. We use bacterial nanocellulose off-the-shelf grafts and describe a detailed methodology to study the ability of these grafts to re-colonize with endothelial cells in an in vitro bioreactor model. The viability of the constructs generated in this process was investigated using established cell culture and tissue engineering methods, which includes WST-1 proliferation assay, AcLDL uptake assay, lactate balancing and histological characterization. The data generated this straight forward methodology allow an initial assessment of the principal prospects of success in forming a stable endothelium in artificial vascular prostheses.

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Acknowledgments

This project was funded by the German Research Foundation (grant number WA 4489/1-1).

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Author notes

  1. Max Wacker and Jan Riedel contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiothoracic Surgery, University Hospital Magdeburg, Magdeburg, Germany

    Max Wacker, Jan Riedel, Priya Veluswamy, Maximilian Scherner, Jens Wippermann & Jörn Hülsmann

  2. Core Facility Tissue Engineering, Otto-von-Guericke University Magdeburg, Magdeburg, Germany

    Heike Walles

Authors
  1. Max Wacker
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  2. Jan Riedel
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  3. Priya Veluswamy
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  4. Maximilian Scherner
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  5. Jens Wippermann
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  6. Heike Walles
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  7. Jörn Hülsmann
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Corresponding author

Correspondence to Max Wacker .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Wacker, M. et al. (2021). Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model. In: Turksen, K. (eds) Bioreactors in Stem Cell Biology. Methods in Molecular Biology, vol 2436. Humana, New York, NY. https://doi.org/10.1007/7651_2021_417

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  • DOI: https://doi.org/10.1007/7651_2021_417

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2017-5

  • Online ISBN: 978-1-0716-2018-2

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