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Scratch Wound Healing Assay

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Epidermal Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2109))

Abstract

Cell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis.

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References

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Author information

Authors and Affiliations

  1. DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Vercelli, Italy

    Simona Martinotti & Elia Ranzato

Authors
  1. Simona Martinotti
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  2. Elia Ranzato
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Corresponding author

Correspondence to Elia Ranzato .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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© 2019 Springer Science+Business Media New York

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Martinotti, S., Ranzato, E. (2019). Scratch Wound Healing Assay. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY. https://doi.org/10.1007/7651_2019_259

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  • DOI: https://doi.org/10.1007/7651_2019_259

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0250-8

  • Online ISBN: 978-1-0716-0251-5

  • eBook Packages: Springer Protocols

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