Abstract
The application of immunohistological methods provides an invaluable contribution in revealing the protein colocalization, which may reflect the occurrence of molecular interaction processes.
This chapter describes comprehensive protocols for detection of S100A7/JAB1 colocalization by immunohistochemistry in archival formalin-fixed and paraffin-embedded skin biopsies from healthy and psoriatic subjects. In addition, we provide a protocol for immunocytochemical detection of S100A7/JAB1 colocalization in S100A7 CRISPR-activated human keratinocyte cell line.
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References
Boehncke WH, Schön MP (2015) Psoriasis. Lancet 386:983–994
Nestle FO, Kaplan DH, Barker J (2009) Psoriasis. N Engl J Med 361:496–509
D’Amico F, Skarmoutsou E, Granata M, Trovato C, Rossi GA, Mazzarino MC (2016) S100A7: a rAMPing up AMP molecule in psoriasis. Cytokine Growth Factor Rev 32:97–104
Hagens G, Roulin K, Hotz R, Saurat JH, Hellman U, Siegenthaler G (1999) Probable interaction between S100A7 and E-FABP in the cytosol of human keratinocytes from psoriatic scales. Mol Cell Biochem 192:123–128
Emberley ED, Gietz RD, Campbell JD, HayGlass KT, Murphy LC, Watson PH (2002) RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer. BMC Cancer 2:28
Emberley ED, Niu Y, Leygue E, Tomes L, Gietz RD, Murphy LC, Watson PH (2003) Psoriasin interacts with Jab1 and influences breast cancer progression. Cancer Res 63:1954–1961
Wei N, Deng XW (2003) The COP9 signalosome. Annu Rev Cell Dev Biol 19:261–286
Emberley ED, Niu Y, Curtis L, Troup S, Mandal SK, Myers JN, Gibson SB, Murphy LC, Watson PH (2005) The S100A7-c-Jun activation domain binding protein 1 pathway enhances prosurvival pathways in breast cancer. Cancer Res 65:5696–5702
Granata M, Skarmoutsou E, Gangemi P, Mazzarino MC, D’Amico F (2019) S100A7, Jab1, and p27kip1 expression in psoriasis and S100A7 CRISPR-activated human keratinocyte cell line. J Cell Biochem 120:3384–3392
Granata M, Skarmoutsou E, Mazzarino MC, D’Amico F (2019) S100A7 in psoriasis: immunodetection and activation by CRISPR technology. In: Heizmann CW (Ed) Calcium-binding proteins of the EF-hand superfamily. Methods in molecular biology. Humana Press, New York, pp 729–738
Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675
Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez JY, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676–682
Ruifrok AC, Johnston DA (2001) Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol 23:291–299
Varghese F, Bukhari AB, Malhotra R, De A (2014) IHC Profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples. PLoS One 9:e96801
Acknowledgments
This work was supported by intramural grants from the University of Catania, Italy.
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Granata, M., Skarmoutsou, E., Mazzarino, M.C., Libra, M., D’Amico, F. (2019). Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY. https://doi.org/10.1007/7651_2019_251
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DOI: https://doi.org/10.1007/7651_2019_251
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