Abstract
A new pancreas cancer cell line, SUIT-58, was established from metastatic liver tumor. The cultured cells exhibited polygonal shape, and proliferated in a form of sheet-structure showing prominent nucleoli and frequent mitotic features. Chromosome count ranged from 54 to 73 with modal chromosome numbers 72 and 73. It was noteworthy that this cell line grew in the serum-free media and maintained in this condition for 30 passages (designated as S58-SF). Both SUIT-58 and S58-SF cell lines were successfully transplanted into nude mice, and their tumor doubling times in xenografts were calculated as 5.4 and 2.8 days, respectively. Histopathologically, the xenografts formed glandular structure that resembled the original tumor. In culture media, the doubling time of SUIT-58 and S58-SF cell lines was calculated as 32 and 35.7 h, respectively. Although the cellular arrangements of SUIT-58 and S58-SF cell lines are different to some extent, their subcellular structures under electron microscope were similar with a large number of lysosomes and distinct desmosomes at cell-cell adhesion sites. The present SUIT-58 and its derivative cell line S58-SF will be applicable for biological studies to develop a new clinical treatment of refractory pancreatic cancer.
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Acknowledgments
We thank Drs. Hiroaki Kataoka and Kenji Yorita (The Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine, University of Miyazaki) for their expertise on pathological diagnosis, Mr. Yoshiteru Goto (Division of Bio-Imaging Laboratory in Frontier Science Research Center, University of Miyazaki) and Ms. Yasuyo Todaka (Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki) for their expert assistances. This study was supported by Grants-in-Aids for Scientific Research from the Japan Society for the Promotion of Science (No. 23791552).
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Takahashi, N., Aoyama, F., Ohuchida, J. et al. Establishment and characterization of SUIT-58 pancreas cancer cell line and its subline S58-SF adapted to serum-free condition derived from metastatic liver tumor. Human Cell 28, 190–198 (2015). https://doi.org/10.1007/s13577-015-0122-6
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DOI: https://doi.org/10.1007/s13577-015-0122-6