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Mycoplasma pneumoniae in Community-Acquired Lower Respiratory Tract Infections

  • Original Article
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Abstract

Objective

To determine the role of Mycoplasma pneumoniae (M. pneumoniae) in pediatric lower respiratory tract infections (LRTIs) employing serological tests and polymerase chain reaction (PCR) analysis.

Methods

In this prospective study, 200 children aged 6 mo to 12 y hospitalized with acute LRTIs were investigated for M. pneumoniae. Serum samples were collected for serological analysis of M. pneumoniae. Throat swab samples were obtained on admission to amplify 277-base pair region of 16S rDNA gene of M. pneumoniae by PCR.

Results

In the present study, 40(26.1%) children <5 y and 28(59.5%) children ≥5 y age group were positive for M. pneumoniae infection and this difference was statistically significant (P < 0.001). M. pneumoniae was positive in 32(41%) female and 36(29.5%) male children though this difference was statistically insignificant (P = 0.12). The clinical profile across M. pneumoniae positive and negative cases were comparable except for presence of chest pain which was statistically significant (P = 0.023). None of the radiological findings was statistically associated with incidence of M. pneumoniae infection. Serological evidence of acute M. pneumoniae infection was observed in 64(32%) patients with sensitivity 66.6% and specificity 70.1% while PCR positivity in 12(6%) patients with sensitivity 12.5% and specificity 97%. Together, serology and PCR detected M.pneumoniae infection in 68(34%) patients.

Conclusions

The present study underlines the role of M. pneumoniae in children with community- acquired LRTIs and more particularly in ≥5 y of age.

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References

  1. Kumar S, Saigal SR, Sethi GR. Detection of IgM and IgG antibodies to Chlamydophila pneumoniae in pediatric community-acquired lower respiratory tract infections. Indian J Pathol Microbiol. 2011;54:782–5.

    PubMed  Google Scholar 

  2. Kogoj R, Mrvic T, Praprotnik M, Kese D. Prevalence, genotyping and macrolide resistance of Mycoplasma pneumoniae among isolates of patients with respiratory tract infections, Central Slovenia, 2006 to 2014. Euro Surveill. 2015;20 https://doi.org/10.2807/1560-7917.ES.2015.20.37.30018.

  3. Cao B, Zhao CJ, Yin YD, et al. High prevalence of macrolide resistance in Mycoplasma pneumoniae isolates from adult and adolescent patients with respiratory tract infection in China. Clin Infect Dis. 2010;51:189–94.

    Article  CAS  PubMed  Google Scholar 

  4. Zhao F, Lv M, Tao X, et al. Antibiotic sensitivity of 40 Mycoplasma pneumoniae isolates and molecular analyis of macrolide resistant isolates from Beijing, China. Antimicrob Agents Chemother. 2012;56:1108–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Waites KB, Talkington DF. Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev. 2004;17:697–728.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Williamson J, Marmion BP, Worswick DA, et al. Laboratory diagnosis of Mycoplasma pneumoniae. Antigen capture and PCR-gene amplification for detection of the mycoplasma: problems of clinical correlation. Epidemiol Infect. 1992;109:519–37.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Tjhie JH, van Kuppeveld FJ, Roosendaal R, et al. Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections. J Clin Microbiol. 1994;32:11–6.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Hadi N, Kashef S, Moazzen M, Pour MS, Rezai N. Survey of Mycoplasma pneumoniae in Iranian children with acute lower respiratory tract infections. Braz J Infect Dis. 2011;15:97–101.

    Article  PubMed  Google Scholar 

  9. Defilippi A, Silvestri M, Tacchella A, et al. Epidemiology and clinical features of Mycoplasma pneumoniae infection in children. Respir Med. 2008;102:1762–8.

    Article  PubMed  Google Scholar 

  10. Onuzuka D, Hashizume M, Hagihara A. Impact of weather factors on Mycoplasma pneumoniae pneumonia. Thorax. 2009;64:507–11.

    Article  Google Scholar 

  11. Kumar S, Saigal SR, Sethi GR. Rapid diagnosis of Mycoplasma pneumoniae by polymerase chain reaction in community- acquired lower respiratory tract infections. Trop Doct. 2011;41:160–2.

  12. Heiskanen-Kosma T, Korppi M, Jokinen C, et al. Etiology of childhood pneumonia: serologic results of a prospective, population-based study. Pediatr Infect Dis J. 1998;17:986–91.

    Article  CAS  PubMed  Google Scholar 

  13. Vervloet LA, Camargos PA, Soares DR, Oliveira GA, Oliveira JN. Clinical, radiographic and haematological characterstics of Mycoplasma pnumoniae pneumonia. J Pediatr. 2010;86:480–7.

    Article  Google Scholar 

  14. Ferwerda A, Moll HA, de Groot R. Respiratory tract infections by Mycoplasma pneumoniae in children: a review of diagnostic and therapeutic measures. Eur J Pediatr. 2001;160:483–91.

    Article  CAS  PubMed  Google Scholar 

  15. Kashyap B, Kumar S, Sethi GR, Das BC, Saigal SR. Comparison of PCR, culture and serological tests for the diagnosis of Mycoplasma pneumoniae in community- acquired lower respiratory tract infections in children. Indian J Med Res. 2008;128:134–9.

    CAS  PubMed  Google Scholar 

  16. Maheshwari M, Kumar S, Sethi GR, Bhalla P. Detection of Mycoplasma pneumoniae in children with lower respiratory tract infections. Trop Doct. 2011;41:40–2.

  17. Lieberman D, Schlaeffer F, Lieberman D, Horowitz S, Horovitz V, Porath A. Mycoplasma pneumoniae community-acquired pneumonia: a review of 101 hospitalized adult patients. Respiration. 1996;63:261–6.

    Article  CAS  PubMed  Google Scholar 

  18. Principi N, Esposito S, Blasi F, Allegra L; The Mowgli Study Group. Role of Mycoplasma pneumoniae and Chlamydia pneumoniae in children with community-acquired lower respiratory tract infections. Clin Infect Dis. 2001;32:1281–9.

  19. Deerojanawong J, Prapphal N, Suwanjutha S, Lochindarat S, Chantarojanasiri T, Kunakorn M. Prevalence and clinical features of Mycoplasma pneumoniae in Thai children. J Med Assoc Thail. 2006;89:1641–7.

    Google Scholar 

  20. Petitjean-Lecherbonnier J, Vabret A, Gouarin S, Dina J, Legrand L, Freymuth F. Mycoplasma pneumoniae infections: retrospective study in Basse-Normandie, 1997–2005, epidemiology–diagnostic utility of serology and PCR for a rapid diagnostic. Pathol Biol. 2006;54:603–11.

    Article  CAS  PubMed  Google Scholar 

  21. Atkinson TP, Balish MF, Waites KB. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev. 2008;32:956–73.

    Article  CAS  PubMed  Google Scholar 

  22. Loens K, Ieven M. Mycoplasma pneumoniae: current knowledge on nucleic acid amplification techniques and serological diagnostics. Front Microbiol. 2016;7:448.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Beersma MFC, Dirven K, van Dam AP, Templeton KE, Class ECJ, Goosens H. Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae specific immunoglobulin G(IgG) and IgM antibodies, with PCR used as the gold standard. J Clin Microbiol. 2005;43:2277–85.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Dorigo-Zetsma JW, Zaat SAJ, Wertheim- van Dillen PME, et al. Comparison of PCR, culture and serological tests for diagnosis of Mycoplasma pneumoniae respiratory tract infection in children. J Clin Microbiol. 1999;37:14–7.

    CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Maulana Azad Medical College, New Delhi, 110002, India

    Surinder Kumar & Indu Bala Garg

  2. Department of Pediatrics, Maulana Azad Medical College, New Delhi, India

    Gulshan Rai Sethi

Authors
  1. Surinder Kumar
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  2. Indu Bala Garg
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  3. Gulshan Rai Sethi
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Contributions

SK: Conceptualized and designed the study, designed data collection instruments, collected data, carried out initial analyses, drafted the initial manuscript, and approved the final manuscript as submitted; IBG and GRS: carried out the further analyses, reviewed and revised the manuscript, and approved the final manuscript as submitted. SK will act as guarantor for this paper.

Corresponding author

Correspondence to Surinder Kumar.

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None.

Source of Funding

University Grants Commission, Bahadurshah Zafar Marg, New Delhi, India, sanctioned the financial assistance for this major research project vide sanction letter No. F.No. 32–575/2006(SR), dated: 07–03-2007.

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Kumar, S., Garg, I.B. & Sethi, G.R. Mycoplasma pneumoniae in Community-Acquired Lower Respiratory Tract Infections. Indian J Pediatr 85, 415–419 (2018). https://doi.org/10.1007/s12098-017-2580-1

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  • DOI: https://doi.org/10.1007/s12098-017-2580-1

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