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Cytogenetic and ITS-psbA-trnH Sequence Analysis for Phylogenetic Inference in Mucuna sp. of India

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Abstract

This paper reports new chromosomal information and earliest proof on efficacy of nrITS and cp-psbA-trnH gene sequences for barcoding and phylogenetic studies in Mucuna sp. First cytological evidence on M. sempervirens and M. bracteata revealed symmetrical karyotype in both the species. Meiotic anomalies evident from the formation of chromosomal laggards and bridges in M. gigantea and M. atropurpurea and unreduced pollens in M. bracteata suggest evolutionary events that might have shaped annual species in this genus. Molecular investigations using nrITS and cp-psbA-trnH sequences revealed them to be phylogenetically informative. Vis-à-vis individual genes, combined sequences of ITS and psbA-trnH sequences offered reliable data for species delineation. The results summarized here are expected to galvanize molecular taxonomic studies and open-up newer means for answering phylogenetic questions in this genus.

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References

  • A’lvarez I, Wendel JF (2003) Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 29:417–434

    Article  Google Scholar 

  • Agostini K, Sazima M, Tozzi AMGA, Forni-Martins ER (2009) Microsporogenesis and pollen morphology of Mucuna japira Azevedu, Agostini and Sazima and M. urens (L.) Medikus. Phytomorphology 59(1 & 2):61–69

    Google Scholar 

  • Aitawade M, Makarand, Yadav SR (2012) Mucuna sanjappae, a new species from the North-Western Ghats, India. Kew Bull 67(3):539–543

    Article  Google Scholar 

  • Bressani R (2002) Factors influencing nutritive value in food grain legumes: Mucuna compared to other grain legumes. In: Flores BM, Eilitta M, Myhrman R, Carew LB, Carsky RJ (eds) Food and feed from Mucuna: current uses and the way forward. Workshop, CIDICCO, CIEPCA and World Hunger Research Center, Tegucigalpa, pp 164–188

    Google Scholar 

  • Buckles D (1995) Velvetbean: a “new” plant with a history. Eco Bot 49:13–25

    Article  Google Scholar 

  • Capo-chichi LJA, Weaver DB, Morton CM (2001) AFLP assessment of genetic variability among Velvetbean (Mucuna sp.) accessions. Theor Appl Genet 103:1180–1188

    Article  CAS  Google Scholar 

  • Carsky RJ, Ndikawa R (1998) Identification of cover crops for the semi-arid savanna zone of West Africa. In: Buckles D, Eteka A, Osiname M, Galiba M, Galiano G (eds) Cover crops in West Africa - contributing to sustainable agriculture. IDRC, IITA, Sasakawa Global 2000, Otawa, pp 179–187

    Google Scholar 

  • Cecil Saldanha J (1996) Flora of Karnataka, ISBN 81-204-1040-8

  • Dassanayake MD, Fosberg FR (1980) A revised handbook to the flora of Ceylon. Vol I

  • Delgado-Salinas A, Bibler R, Lavin M (2006) Phylogeny of the genus Phaseolus (Leguminosae): a recent diversification in an ancient landscape. Syst Bot 31:779–791

    Article  Google Scholar 

  • Eilitta M, Bressani R, Carew LB, Carsky RJ, Flores M, Gilbert R, Huyck L, St Laurent L, Szabo NJ (2002) Mucuna as a food and feed crop: an overview. In: Flores BM, Eilittä M, Myhrman R, Carew LB, Carsky RJ (eds) Food and feed from Mucuna: current uses and the way forward. Workshop, CIDICCO, CIEPCA and World Hunger Research Center, Tegucigalpa, pp 18–47

    Google Scholar 

  • Ellis L (1990) Flora of Nallamalais 2:220–490

    Google Scholar 

  • Farooqi AA, Khan MM, Asundhara M (1999) Production technology of medicinal and aromatic crops. Natural Remedies Pvt. Ltd, Bangalore, pp 26–28

    Google Scholar 

  • Farris JS (1989a) The retention index and homoplasy excess. Syst Zool 38:406–407

    Article  Google Scholar 

  • Farris JS (1989b) The retention index and the rescaled consistency index. Cladistics 5:417–419

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Goel S, Raina SN, Ogihara Y (2002) Molecular evaluation and phylogenetic implication of internal transcribed spacer sequences of nuclear ribosomal DNA in the Phaseolus-Vigna complex. Mol Phylo and Evol 22(1):1–19

    Article  CAS  Google Scholar 

  • Jaheer M, Sathyanarayana N (2010) Karyomorphological studies in Mucuna of India. Chromosome Bot 5:37–41

    Article  Google Scholar 

  • Jorge MA, Eilitta M, Proud FJ, Maasdorp B, Beksissa VH, Sarial AK, Hanson J (2007) Mucuna species: recent advances in application of biotechnology. Fruit Veg Cer  Sci Biotech 2:80–94

  • Kluge AG, Farris JS (1969) Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32

    Article  Google Scholar 

  • Kress WJ, Wurdack KJ, Zimmer EA, Weight LA, Janzen DH (2005) Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci U S A 102:8369–8374

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Lavin M, Doyle JJ, Palmer JD (1990) Evolutionary significance of the loss of the chloroplast-DNA inverted repeat in the Leguminoceae subfamily Papilionoideae. Evol 44(2):390–402

    Article  CAS  Google Scholar 

  • Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220

    Article  Google Scholar 

  • Lima De Faria A (1980) Classification of genes, rearrangements and chromosomes according to the field. Hereditas 93:1–46

    Article  CAS  PubMed  Google Scholar 

  • Mercado-Ruaro P, Delgado-Salinas A (1998) Karyotypic studies on species of Phaseolus (Fabaceae: Phaseolinae). Amer J of Bot 85:1–9

    Article  CAS  Google Scholar 

  • Palmer JD, Nugent JM, Herbon LA (1987) Unusual structure of geranium chloroplast DNA: a triplesized inverted repeat, extensive gene duplications, multiple inversions, and two repeat families. Proc Natl Acad Sci U S A 84:769–773

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Rajaram N, Janardhanan K (1991) The biochemical composition and nutritional potential of the tribal pulse, Mucuna gigantea (Wild) DC. Plant Foods Hum Nutr 41:45–51

    Article  CAS  PubMed  Google Scholar 

  • Renaud L, Bank M van der, Bogarin D, Warner J, Pupulin F, Guillau.me Gigot, Maurin O, Duthoit S, Barraclough TG, Savolainen V (2008) DNA barcoding the floras of biodiversity hotspots. Ecology. Early Edition 1 of 6

  • Sasidharan (2004) Biodiversity documentation for Kerala part 6: flowering plants

  • Sastrapradja S, Sastrapradja D, Aminah SH, Lubis I, Idris S (1974) Morphological and cytological investigation on some species of Mucuna (Papilionaceae). Annals Bogorienses 5(4):173–178

    Google Scholar 

  • Shaw J, Lickey EB, Schilling EE, Small RL (2007) Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms. The tortoise and the hare III. Amer J Bot 94:275–288

    Article  CAS  Google Scholar 

  • Souza MG, Benko-Iseppon AM (2004) Cytogenetics and chromosome banding patterns in Caesalpinioideae and Papilionioideae species of Pará, Amazonas. Brazil Bot J Linn Soc 144:181–191

    Article  Google Scholar 

  • Stebbins GL (1958) Longevity, habitat and release of genetic variability in higher plants. Cold Spring Harb Symp Quant Biol 23:365–377

    Article  CAS  PubMed  Google Scholar 

  • Stefanovic S, Bernard EP, Jeffrey DP, Jeff JD (2009) Relationship among phaseoloid legumes based on sequences from eight chloroplast regions. Syst Bot 34(1):115–128

    Article  Google Scholar 

  • Suguira M (1992) The chloroplast genome. Plant Mol Biol 19:149–168

    Article  Google Scholar 

  • Swofford DL (2011) PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol and Evol 24:1596–1599

    Article  CAS  Google Scholar 

  • Tao Sang DJ, Crawford TFS (1997) Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Amer J of Bot 84(9):1120–1136

    Article  Google Scholar 

  • Tarawali G, Manyong VM, Carsky RJ, Vissoh PV, Osei-Bonsu P, Galiba M (1999) Adoption of improved fallows in West Africa: lessons from Mucuna and Stylo case studies. Agroforestry Syst 47:93–122

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Wilmot-Dear CM (1987) A revision of Mucuna (Leguminosae, Phaseoleae) in the Indian subcontinent and Burma. Kew Bull 42(1):23–46

    Article  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the financial support from

1. Department of Science and Technology (DST), GOI; Grant number: SR/SO/PS/0028/2011.

2. Dept. of Biotechnology (DBT), GOI. Grant number: BT/PR3489/PBD/16/945/2011

3. Sri Krishnadevaraya Educational Trust (Sri KET), Bangalore,

4. Sikkim University, Gangtok.

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    Authors and Affiliations

    1. Department of Biotechnology, Sir M Visvesvaraya Institute of Technology, Bangalore, India

      M. Jaheer, Deepika Bhat & K. V. Rashmi

    2. USDA-ARS, Cropping System Research Laboratory, Lubbock, TX, 79415, USA

      R. Chopra

    3. Rubicon Genomics, Inc, Ann Arbor, MI, USA

      K. R. Kunder

    4. Department of Botany, Sikkim University, 6th Mile, Samdur, Tadong, 737 102, Gangtok, India

      N. Sathyanarayana

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    1. M. Jaheer
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    Correspondence to N. Sathyanarayana.

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    Additional information

    Communicated by: Paulo Arruda

    KKR completed this research when she was student of MVIT

    R. Chopra and K. R. Kunder contributed equally to this work.

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    Jaheer, M., Chopra, R., Kunder, K.R. et al. Cytogenetic and ITS-psbA-trnH Sequence Analysis for Phylogenetic Inference in Mucuna sp. of India. Tropical Plant Biol. 8, 108–116 (2015). https://doi.org/10.1007/s12042-015-9154-z

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