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Somatic embryogenesis of a seedless sweet orange (Citrus sinensis (L.) Osbeck)

  • Plant Tissue Culture
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A Correction to this article was published on 17 January 2018

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Abstract

Somatic embryogenesis is an important in vitro technique used to obtain Citrus sinensis (L.) Osbeck (sweet orange) plantlets for conservation, sanitation, propagation, and breeding. The induction of somatic embryogenesis from adult tissues of sweet orange could be an alternative to in vitro organogenesis from epicotyl segments, especially in seedless cultivars, where the latter is not feasible. The aim of this study was to obtain plantlets from ovary-derived somatic embryos of sweet orange cv. ‘Washington Navel’, an important seedless cultivar for citrus fresh fruit production. The explants used were pistils from flower buds, pre-anthesis, from 20-y-old plants cultivated in the field. Forty plantlets from 47 somatic embryos were obtained, in vitro-grafted, and acclimatized in greenhouse conditions. Ploidy evaluation through flow cytometric analysis, as well as the results of target region amplification polymorphism (TRAP) molecular markers confirmed the somatic origin of embryos as genetically similar to donor plants. This technique could be used for obtaining embryogenic cell suspension cultures or regenerated plants from mature tissues other than seed-derived tissues, especially for seedless genotypes.

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  • 17 January 2018

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Funding

JCC and RRL acknowledge Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for the research funds and a doctorate scholarship for JCC (Process number 2008/09928-8).

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Authors and Affiliations

  1. Department of Biotechnology, Plant and Animal Production, Centro de Ciências Agrárias, UFSCar, Rodovia Anhanguera, km 174, CP 153, Araras, SP, 13600-970, Brazil

    Jean C. Cardoso

  2. Universidade de São Paulo, CENA, Av. Centenário, 303, Piracicaba, SP, 13416-903, Brazil

    Jean C. Cardoso & Adriana P. Martinelli

  3. Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP, Brazil

    Maisa Curtolo

  4. Centro APTA Citros “Sylvio Moreira”/IAC, Cordeirópolis, SP, Brazil

    Rodrigo R. Latado

Authors
  1. Jean C. Cardoso
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  2. Maisa Curtolo
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  3. Rodrigo R. Latado
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  4. Adriana P. Martinelli
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Contributions

JCC conceptualized, designed, and performed the experiments, data analysis, drafting, and editing of the manuscript. MC contributed with molecular analysis and evaluation of experiments. RRL was responsible for conception of molecular analysis, lab infrastructure, and review of the manuscript. APM oversaw the study design and critically reviewed and edited the manuscript. All authors read and agreed with the submission of the manuscript.

Corresponding author

Correspondence to Jean C. Cardoso.

Additional information

Editor: Jude Grosser

A correction to this article is available online at https://doi.org/10.1007/s11627-018-9886-1.

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Cardoso, J.C., Curtolo, M., Latado, R.R. et al. Somatic embryogenesis of a seedless sweet orange (Citrus sinensis (L.) Osbeck). In Vitro Cell.Dev.Biol.-Plant 53, 619–623 (2017). https://doi.org/10.1007/s11627-017-9866-x

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  • DOI: https://doi.org/10.1007/s11627-017-9866-x

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