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Establishment and biological characteristics comparison of Chinese swamp buffalo (Bubalus bubalis) fibroblast cell lines

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Abstract

To establish fibroblast cell lines from different tissues and to compare the biological characteristics of those cell lines, five fibroblast cell lines derived from Chinese swamp buffalo (Bubalus bubalis) were selected for comparative assays. Cell style and survival rate (before cryogenic preservation and after recovery) were tested, and karyotype, patterns of isoenzymes of lactic dehydrogenase, malic dehydrogenase, and cell cycle were analyzed. These cell lines had a healthy morphology with a typical spindle shape, and assessment of cell style showed these cells to be very pure fibroblasts. Cell growth curves showed a typical “S” shape. Results of microorganism contamination assays were negative, and isoenzyme analysis showed no cross-contamination. The number of chromosomes (2n) of swamp buffalo is 48. Between 28% and 46% of the cells were 2n, and cell apoptosis was not pronounced at 20th generation. Results showed that skin fibroblasts were more adaptable to tissue culture conditions than the ones from kidneys and ear margin, and they are more suitable for cellular manipulation in Chinese swamp buffalo.

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Acknowledgements

This study was supported by Guangxi Science Foundation (2010GXNSFF013002) and the Open Fund of State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources (Grant SB1001).

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China

    Jun Luo, Ming-ming Liang, Xiao-gan Yang, Hui-yan Xu, De-shun Shi & Sheng-sheng Lu

  2. College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China

    Jun Luo

  3. College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China

    Ming-ming Liang, Xiao-gan Yang, Hui-yan Xu, De-shun Shi & Sheng-sheng Lu

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  1. Jun Luo
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  2. Ming-ming Liang
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  4. Hui-yan Xu
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  5. De-shun Shi
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  6. Sheng-sheng Lu
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Correspondence to Sheng-sheng Lu.

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Editor: T. Okamoto

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Luo, J., Liang, Mm., Yang, Xg. et al. Establishment and biological characteristics comparison of Chinese swamp buffalo (Bubalus bubalis) fibroblast cell lines. In Vitro Cell.Dev.Biol.-Animal 50, 7–15 (2014). https://doi.org/10.1007/s11626-013-9677-2

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  • DOI: https://doi.org/10.1007/s11626-013-9677-2

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