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Kinetic analysis of interaction between lipopolysaccharide and biomolecules

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Frontiers of Chemistry in China

Abstract

Lipopolysaccharide (LPS) is a major component of the outer membrane of all gram-negative bacteria. It interacts with some biomolecules and triggers a toxic reaction. In this paper, we studied the interaction between LPS from Salmonella Minnesota and some biomolecules using a surface plasmon resonance (SPR) biosensor. Biomolecules were immobilized on a CM5 sensor chip using the amino coupling method and LPS was injected over the immobilized surfaces. The affinity constant KA of LPS with serum albumin, hemoglobin, chitosan and lysozyme was 2.36 × 107, 2.03 × 108, 7.58 × 106, 2.82 × 104 L·mol−1, respectively. However, LPS could not interact with ferritin.

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Fan Yang & Xiurong Yang

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  1. Fan Yang
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  2. Xiurong Yang
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Correspondence to Xiurong Yang.

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Translated from Chinese Journal of Analytical Chemistry, 2007, 35(5): 677–680 [译自: 分析化学]

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Yang, F., Yang, X. Kinetic analysis of interaction between lipopolysaccharide and biomolecules. Front. Chem. China 3, 14–17 (2008). https://doi.org/10.1007/s11458-008-0005-4

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  • DOI: https://doi.org/10.1007/s11458-008-0005-4

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