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Morus alba leaf extract mediates neuroprotection against glyphosate-induced toxicity and biochemical alterations in the brain

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Abstract

Recent studies demonstrate that glyphosate exposure is associated with oxidative stress and some neurological disorders such as Parkinson’s pathology. Therefore, phytochemicals, in particular phenolic compounds, have attracted increasing attention as potential agents for neuroprotection. In the present study, we investigate the impact of glyphosate on the rat brain following i.p. injection and the possible molecular target of neuroprotective activity of the phenolic fraction from Morus alba leaf extract (MALE) and its ability to reduce oxidative damage in the brain. Wistar rats from 180 to 240 g were i.p. treated with a single dose of glyphosate (100 mg kg−1 b.w.) or MALE (100 μg mL−1 kg−1 b.w.) for 2 weeks. Brain homogenates were used to evaluate neurotoxicity induced by the pesticide. For this, biochemical parameters were measured. Data shows that MALE regulated oxidative stress and counteracted glyphosate-induced deleterious effects and oxidative damage in the brain, as it abrogated LDH, protein carbonyls, and malonyldialdehyde. MALE also appears to be able to scavenge H2O2 levels, maintain iron and Ca2+ homeostasis, and increase SOD activity. Thus, in vivo results showed that mulberry leaf extract is a potent protector against glyphosate-induced toxicity, and its protective effect could result from synergism or antagonism between the various bioactive phenolic compounds in the acetonic fraction from M. alba leaf extract.

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Abbreviations

MALE:

Morus alba leaf extract

b.w:

Body weight

i.p:

Intra-peritonial

SOD:

Superoxide dismutase

LDH:

Lactate dehydrogenase

AMPA:

Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate

PD:

Parkinson’s disease

POD:

Peroxidase

MDA:

Malondialdehyde

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Acknowledgements

This study was supported by the Research Unit 00-UR-08-01. We gratefully acknowledge the financial support of the Tunisian Ministry of High Education, Scientific Research and Technology.

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Authors and Affiliations

  1. Research Unit of Functional Neurophysiology and Pathology, 00/UR/08-01, Faculty of Science of Tunis, University El Manar, 2092, Tunis, Tunisia

    Olfa Rebai, Manel Belkhir & Mohamed Amri

  2. Laboratory LIP-MB, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Tunis, Tunisia

    Adnen Boujelben & Sami Fattouch

Authors
  1. Olfa Rebai
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  2. Manel Belkhir
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  3. Adnen Boujelben
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  4. Sami Fattouch
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  5. Mohamed Amri
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Correspondence to Olfa Rebai.

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Responsible editor: Philippe Garrigues

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Rebai, O., Belkhir, M., Boujelben, A. et al. Morus alba leaf extract mediates neuroprotection against glyphosate-induced toxicity and biochemical alterations in the brain. Environ Sci Pollut Res 24, 9605–9613 (2017). https://doi.org/10.1007/s11356-017-8584-6

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  • DOI: https://doi.org/10.1007/s11356-017-8584-6

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