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Elicitor-enhanced syringin production in suspension cultures of Saussurea medusa

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Abstract

Syringin production and related secondary metabolism enzyme activities in suspension cultures of Saussurea medusa treated with different elicitors (yeast extract, chitosan and Ag+) were investigated. All elicitors enhanced syringin production, and the optimal feeding protocol was the combined addition of 1.5% (v/v) yeast extract, 0.2 g l−1 chitosan and 75 μM Ag+ at the 15th day of the cell culture. The highest syringin production reached 741.9 mg l−1, which was 3.6−fold that of the control. The glucose−6-phosphate dehydrogenase (EC 1.1.1.49), phenylalanine ammonia lyase (EC 4.3.1.5) and peroxidase (EC 1.11.1.7) activities increased significantly after elicitor treatment. The maximum enzyme activities were obtained when the treatment time was 6 h.

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Abbreviations

6BA:

6-Benzylaminopurine

DTT:

Dithiotreitol

G6PDH:

Glucose-6-phosphate dehydrogenase

NAA:

α-Naphthaleneacetic acid

PAL:

Phenylalanine ammonia lyase

POD:

Peroxidase

PPP:

Pentose phosphate pathway

PVPP:

Polyvinyl polypyrrolidone

ROS:

Reactive oxygen species

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Acknowledgment

This research is supported by Directional Project of Knowledge Innovation Engineering, CAS (KGCX2-SW−506).

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Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China

    Chunming Xu, Bing Zhao, Yuan Ou, Xiaodong Wang, Xiaofan Yuan & Yuchun Wang

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Chunming Xu & Yuan Ou

Authors
  1. Chunming Xu
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  2. Bing Zhao
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  3. Yuan Ou
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  4. Xiaodong Wang
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  5. Xiaofan Yuan
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  6. Yuchun Wang
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Correspondence to Yuchun Wang.

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Xu, C., Zhao, B., Ou, Y. et al. Elicitor-enhanced syringin production in suspension cultures of Saussurea medusa . World J Microbiol Biotechnol 23, 965–970 (2007). https://doi.org/10.1007/s11274-006-9321-3

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  • DOI: https://doi.org/10.1007/s11274-006-9321-3

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