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Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use

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Abstract

Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).

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Acknowledgements

This research received support from the QualityNano Project http://www.QualityNano.eu which is financed by the European Community Research Infrastructures under the F7 Capacities Programme (grant no. INFRA-2010-262163) and its partner University of Namur. This work was also supported by the DGO6 (Direction Générale Opérationnelle de l’Economie, de l’Emploi et de la Recherche) of the Walloon Region of Belgium (“Project Complément FP7 Qnano, Convention n°1117448”).

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Authors and Affiliations

  1. Namur Nanosafety Centre, University of Namur, Namur, Belgium

    Julie Laloy, Lutfiye Alpan, Jorge Mejia, Jean-Michel Dogné & Stéphane Lucas

  2. Department of Pharmacy, NARILIS, University of Namur, Rue de Bruxelles, 61, 5000, Namur, Belgium

    Julie Laloy, Hélène Haguet, Lutfiye Alpan & Jean-Michel Dogné

  3. CHU UCL Namur, NARILIS, Department of Haematology Laboratory, Université Catholique de Louvain, Yvoir, Belgium

    Hélène Haguet

  4. LISM, EA 4695, UFR Sciences Exactes et Naturelles, BP 1039, F-51687, Reims Cedex 2, France

    Valérie Mancier, Samuel Levi, Céline Rousse & Patrick Fricoteaux

  5. Research Centre for the Physics of Matter and Radiation (PMR-LARN), NARILIS, University of Namur, Namur, Belgium

    Jorge Mejia & Stéphane Lucas

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  1. Julie Laloy
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  2. Hélène Haguet
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Correspondence to Julie Laloy.

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Funding

This study was funded by the European Community Research Infrastructures (grant number INFRA-2010-262163) and by the Walloon Region of Belgium (Project Complément FP7 Qnano, Convention n°1117448).

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Laloy, J., Haguet, H., Alpan, L. et al. Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use. J Nanopart Res 19, 266 (2017). https://doi.org/10.1007/s11051-017-3937-0

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