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Association between presence of Triticum timopheevii introgression and D-genome retention in hexaploid/tetraploid wheat crosses

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Abstract

The 2G Triticum timopheevii introgression harbours genes for multiple disease resistance and quality traits in bread wheat. In order to transfer this segment from bread wheat into durum, the bread wheat line Sunguard, which carries this introgressed 2G segment was crossed with three tetraploid durum parents. A significant difference was observed in the segregation ratio of the 2G segment in the different crosses at the F2 generation with two of the three populations indicating segregation distortion against the hexaploid 2G segment. In these populations, the presence of the 2G segment was strongly correlated with the presence of D-genome chromosomes. These results were confirmed in the F4 generation of these populations. Six plants were identified in the F4 generation, which had retained the introgressed 2G segment in a homozygous condition and did not have a complete D-genome set. Two of these lines only had two non-homologous D-genome chromosomes in the F5 generation. Thus, the 2G segment and possibly other translocations can be transferred into durum wheat through hexaploid/tetraploid hybridisation.

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Funding

This study was partly funded by the Grain Research and Development Corporation, Australia. An Australian Postgraduate Award supported Sriram Padmanaban’s Ph.D. study.

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Authors and Affiliations

  1. Centre for Crop Health, University of Southern Queensland, Toowoomba, Queensland, 4350, Australia

    Sriram Padmanaban, Mark W. Sutherland & Anke Martin

  2. Plant Breeding Institute, The University of Sydney, Cobbitty, New South Wales, 2570, Australia

    Peng Zhang

Authors
  1. Sriram Padmanaban
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  2. Peng Zhang
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  3. Mark W. Sutherland
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  4. Anke Martin
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Corresponding author

Correspondence to Anke Martin.

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Electronic supplementary material

Supplementary Table 1

Retention of D-genome chromosomes in 180 F2 progeny of three crosses. Each row represents an individual line and each column represents a non-homologous D chromosome. Red and yellow colours indicate the presence and absence of D chromosomes, respectively. Highlighted lines from each cross were advanced to the F4 generation. Inheritance of the 2G segment in each of the crosses is shown (A = maternal, H = heterozygous and B = paternal genotype) (DOCX 57 kb)

Supplementary Table 2

Microsatellite markers amplified across 2G segment of 26 selected F5 progeny of the Sunguard/Caparoi cross (A = maternal and B = paternal genotype). The estimated locations of the microsatellite markers on chromosome 2B according to consensus map by Apples (2003) are indicated. The number of D chromosomes retained in each of the 26 F5 lines is also given (DOCX 15 kb)

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Padmanaban, S., Zhang, P., Sutherland, M.W. et al. Association between presence of Triticum timopheevii introgression and D-genome retention in hexaploid/tetraploid wheat crosses. Mol Breeding 38, 84 (2018). https://doi.org/10.1007/s11032-018-0838-6

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  • DOI: https://doi.org/10.1007/s11032-018-0838-6

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