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Characterization, drug release profile and cytotoxicity of Dentatin-Hydroxypropyl-β-Cyclodextrin complex

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Abstract

This current work has been conducted mainly to increase solubility and drug release properties for high hydrophobic Dentatin (DEN) by incorporation it into Hydroxypropyl-β-Cyclodextrin (HPβCD) cavity. To confirm that inclusion be succeeded, the produced complex were installed onto different machines. The latter includes: Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and field emission-scanning electron microscopy (FE-SEM). The hydrodynamic diameter and zeta potential of DEN-HPβCD complex were 2.025 ± 0.39 nm and −33.6 mV, respectively. Ultra-violet spectroscopy was employed to further confirmation of complexation process as well as to determine drug release profile. The result showed an initial burst release (19.9% within first two minutes) and then a continuous release for an extended period of 41 h (100%). The solubility of DEN was enhanced by >300 fold following complexation when a compared to DEN alone. Moreover, MTT finding showed that this complexation did not reduce cytotoxicity of DEN after applying on prostate cancer (LNCaP), human adenocarcinoma breast cancer (MDA-MB-231) and human gastric adenocarcinoma cell line (HDT). However, further investigations are required to validate efficacy of our produced inclusion using molecular analysis and in vivo studies.

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Acknowledgements

This research was supported by science fund research grant (02-01-04-sf1210), Ministry of science, Technology and innovation, Malaysia. The author (Al-Abboodi Sh Ashwaq) is grateful to University of AL-Qadisiyah, Ministry of Higher Education and Scientific Research, Iraq.

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Authors and Affiliations

  1. Institute of Bioscience, University Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Al-Abboodi Shakir Ashwaq, Ahmad Bustamam Abdul & Mothanna Sadiq Al-Qubaisi

  2. Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Abdullah Rasedee

  3. Department of Biomedical Science, Faculty of Medicine & Health Science, University Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Ahmad Bustamam Abdul

  4. Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Yun Hin. Taufiq-Yap

  5. Uniazah College of Pharmacy, Qassim University, Buraydah, Saudi Arabia

    Eltayeb E. M. Eid

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  1. Al-Abboodi Shakir Ashwaq
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  2. Abdullah Rasedee
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Correspondence to Al-Abboodi Shakir Ashwaq.

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Ashwaq, AA.S., Rasedee, A., Abdul, A.B. et al. Characterization, drug release profile and cytotoxicity of Dentatin-Hydroxypropyl-β-Cyclodextrin complex. J Incl Phenom Macrocycl Chem 87, 167–178 (2017). https://doi.org/10.1007/s10847-016-0688-y

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