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Microbial kinetics of Clostridium termitidis on cellobiose and glucose for biohydrogen production

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Abstract

Objective

To determine Monod kinetics parameters (µmax, Ks, kd and YX/S) of the mesophilic H2 producer Clostridium termitidis grown on glucose and cellobiose by modeling in MATLAB.

Results

Maximum specific growth rates (µmax) were 0.22 and 0.24 h−1 for glucose and cellobiose respectively; saturation constants (Ks) were 0.17 and 0.38 g l−1 respectively and the biomass yields (YX/S) were 0.26 and 0.257 g dry wt g−1 substrate. H2 yields of 1.99 and 1.11 mol H2 mol−1 hexose equivalent were also determined for glucose and cellobiose respectively.

Conclusion

The microbial kinetics of this model microorganism will enhance engineering biofuel production applications.

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Acknowledgments

This work was supported by the Eastern platform of the Biofuel Network. The authors acknowledge the support by Consejo Nacional de Ciencia y Tecnologia de Mexico (CONACYT) and Alianza para la Formacion e Investigacion en Infraestructura para el Desarrollo de Mexico, awarded to Maritza Gomez-Flores.

Supporting information

Supplementary Figure 1—Correlation between dry wt and cellular protein content in Clostridium termitidis.

Author information

Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Faculty of Engineering, University of Western Ontario, London, ON, N6A 5B9, Canada

    Maritza Gomez-Flores & George Nakhla

  2. Department of Civil and Environmental Engineering, Faculty of Engineering, University of Western Ontario, London, ON, N6A 5B9, Canada

    George Nakhla & Hisham Hafez

Authors
  1. Maritza Gomez-Flores
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  2. George Nakhla
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  3. Hisham Hafez
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Corresponding author

Correspondence to George Nakhla.

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Supplementary material 1 (DOCX 29 kb)

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Gomez-Flores, M., Nakhla, G. & Hafez, H. Microbial kinetics of Clostridium termitidis on cellobiose and glucose for biohydrogen production. Biotechnol Lett 37, 1965–1971 (2015). https://doi.org/10.1007/s10529-015-1891-4

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  • DOI: https://doi.org/10.1007/s10529-015-1891-4

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