We compared the cultivation of human peripheral blood lymphocytes in serum-free medium Hybris-2 and RPMI 1640 medium with 10% fetal bovine serum in the presence of phytohemagglutinin and interleukin-2. The optimal concentration of phytohemagglutinin significantly differed in serum-free and serum-containing media (0.5 and 5 mg/ml, respectively). Both mitogens were more potent in stimulating the proliferation of lymphocytes in serum-free medium than in serum-containing medium. Strong proliferation of CD3+ and CD4+ T lymphocytes was observed in both media. The dynamics of other markers was similar in serum-free and serum-containing media. However, significant differences were revealed between individual donors. Our results indicate that the developed serum-free medium may be used in lymphocyte cultivation for scientific, diagnostic, and therapeutic purposes.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 10-15, 2009
An erratum to this article can be found at http://dx.doi.org/10.1007/s10517-009-0535-x
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Tabakov, V.U., Litvina, M.M., Schepkina, J.V. et al. Studying the Proliferation of Human Peripheral Blood T Lymphocytes in Serum-Free Medium. Bull Exp Biol Med 147, 120–124 (2009). https://doi.org/10.1007/s10517-009-0434-1
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DOI: https://doi.org/10.1007/s10517-009-0434-1