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Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis

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Journal of Industrial Microbiology and Biotechnology

Abstract

A genomic library of biphenyl-degrading Comamonas sp. SMN4 for isolating fragments containing the 2,3-dihydroxybiphenyl 1,2-dioxygenase (23DBDO) gene was constructed. The smallest subclone (pNPX9) encoding 23DBDO activity was sequenced and analyzed. The C-terminal domain of 23DBDO from Comamonas sp. SMN4 had five catalytically essential residues and was more highly conserved than the N-terminal domain. Phylogenetic and structural relationships of 23DBDO from Comamonas sp. SMN4 were analyzed.

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Acknowledgements

This work was partly supported by grant 2000-2-211-002-4 from the Basic Research Program of the Korea Science and Engineering Foundation (KOSEF); N.R. Lee was a post-doctoral fellow supported by KOSEF.

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Authors and Affiliations

  1. Department of Biological Science, Sookmyung Women's University, Chungpa 2-ka, Yongsan, 140–742, Seoul, Korea

    K. H. Min

  2. Korea Food Research Institute, Baekhyon, Poondang, Songnam, 463–746, Kyongki-do, Korea

    N. R. Lee & D. Y. Kwon

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  1. N. R. Lee
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  2. D. Y. Kwon
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  3. K. H. Min
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Correspondence to D. Y. Kwon.

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Lee, N.R., Kwon, D.Y. & Min, K.H. Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis. J IND MICROBIOL BIOTECHNOL 30, 245–250 (2003). https://doi.org/10.1007/s10295-003-0039-z

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  • DOI: https://doi.org/10.1007/s10295-003-0039-z

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