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Optimization of polyphenol extraction and antioxidant activities of extracts from defatted flax seed cake (Linum usitatissimum L.) using microwave-assisted and pulsed electric field (PEF) technologies with response surface methodology

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Abstract

Use of microwave and pulsed electric field (PEF) technologies for optimization of polyphenol extraction from defatted flax seed cake was attempted using a Box-Behnken response surface design. Polyphenols were extracted after microwave or PEF treatments using ultrasound under fixed variables of 200 W of ultrasonic power, a 70°C water bath, and 20 min of extraction time. Measured responses were total phenolic and total flavonoid contents, and 2,2,-diphenyl-1-picrylhydrazyl radical (DPPH·) scavenging activity and ferric reducing/antioxidant power activities. Maximum yields of all responses were achieved under optimum conditions for microwave processing=5 min, a liquid to solid ratio=6, and at 644 W, and for PEF-assisted extraction for 30 V at 30 Hz with a 10% ethanol concentration, and a treatment time=10 s. Under optimized microwave or PEF processing conditions, yields of polyphenols from defatted seed cake were maximized.

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References

  1. Gutiérrez C, Rubilar M, Jara C, Verdugo M, Sineiro J, Shene C. Flaxseed and flaxseed cake as a source of compounds for food industry. J. Soil Sci. Plant Nutr. 10: 454–463 (2010)

    Article  Google Scholar 

  2. Korkina LG. Phenylpropanoids as naturally occurring antioxidants: From plant defense to human health. Cell Mol. Biol. 53: 15–25 (2007)

    CAS  Google Scholar 

  3. Bozan B, Temelli F. Chemical composition and oxidative stability of flax, safflower and poppy seed and seed oils. Bioresource Technol. 99: 6354–6359 (2008)

    Article  CAS  Google Scholar 

  4. Elif BA, Turhan S. Enhancement of the nutritional status of beef patties by adding flaxseed flour. Meat Sci. 82: 472–427 (2009)

    Article  Google Scholar 

  5. Wang L, Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Tech. 17: 300–312 (2006)

    Article  CAS  Google Scholar 

  6. Pan X, Niu G, Liu H. Microwave-assisted extraction of tea polyphenols and tea caffeine from green tea leaves. Chem. Eng. Process. 42: 129–133 (2003)

    Article  CAS  Google Scholar 

  7. Song J, Li D, Liu C, Zhang Y. Optimized microwave-assisted extraction of total phenolics (TP) from Ipomoea batatas leaves and its antioxidant activity. Innov. Food Sci. Emerg. 12: 282–287 (2011)

    Article  CAS  Google Scholar 

  8. Hayat K, Hussain S, Abbas S, Farooq U, Ding B, Xia S, Jia C, Zhang X, Xia W. Optimized microwave-assisted extraction of phenolic acids from citrus mandarin peels and evaluation of antioxidant activity in vitro. Sep. Purif. Technol. 70: 63–70 (2009)

    Article  CAS  Google Scholar 

  9. Angersbach A, Heinz V, Knorr D. Effects of pulsed electric fields on cell membranes in real food systems. Innov. Food Sci. Emerg. 1: 135–149 (2000)

    Article  CAS  Google Scholar 

  10. Maskooki A, Eshtiaghi MN. Impact of pulsed electric field on cell disintegration and mass transfer in sugar beet. Food Bioprod. Process. 90: 377–384 (2012)

    Article  Google Scholar 

  11. Boussetta N, Lanoisellé J-L, Bedel-Cloutour C, Vorobiev E. Extraction of soluble matter from grape pomace by high voltage electrical discharges for polyphenol recovery: Effect of sulphur dioxide and thermal treatments. J. Food Eng. 95: 192–198 (2009)

    Article  CAS  Google Scholar 

  12. Puértolas E, López N, Saldaña G, Álvarez I, Raso J. Evaluation of phenolic extraction during fermentation of red grapes treated by a continuous pulsed electric fields process at pilot-plant scale. J. Food Eng. 98: 120–125 (2010)

    Article  Google Scholar 

  13. Boussetta N, Vorobiev E, Le LH, Cordin-Falcimaigne A, Lanoisellé JL. Application of electrical treatments in alcoholic solvent for polyphenols extraction from grape seeds. LWT-Food Sci. Technol. 46: 127–134 (2012)

    Article  CAS  Google Scholar 

  14. Silva EM, Rogez H, Larondelle Y. Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology. Sep. Purif. Technol. 55: 381–387 (2007)

    Article  CAS  Google Scholar 

  15. Box G, Behnken D. Some new three level designs for the study of quantitative variables. Technometrics 2: 455–475 (1960)

    Article  Google Scholar 

  16. Gutfinger T. Polyphenols in olive oils. J. Am. Oil Chem. Soc. 58: 966–968 (1981)

    Article  CAS  Google Scholar 

  17. Oomah BD, Mazza G, Kenaschuk EO. Flavonoid content of flaxseed. Influence of cultivar and environment. Euphytica 90: 163–167 (1996)

    Article  Google Scholar 

  18. de Ancos B, Sgroppo S, Plaza L, Cano MP. Possible nutritional and healthrelated value promotion in orange juice preserved by high-pressure treatment. J. Sci. Food Agr. 82: 790–796 (2002)

    Article  Google Scholar 

  19. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant power”: The FRAP assay. Anal. Biochem. 239: 70–76 (1996)

    Article  CAS  Google Scholar 

  20. Pinelo M, Fabbro PD, Manzocco L, Nuñez MJ, Nicoli MC. Optimization of continuous phenol extraction from Vitis vinifera by products. Food Chem. 92: 109–117 (2005)

    Article  CAS  Google Scholar 

  21. Chen Y, Xie MY, Gong XF. Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. J. Food Eng. 81: 162–170 (2007)

    Article  CAS  Google Scholar 

  22. Zhou XH, Chen SJ, Chen J, Li MJ, Ai ZL. Study on Optimization of microwave extraction process of polyphenols from rotten jujube peel by using response surface analysis. Food Sci. 29: 265–268 (2008)

    CAS  Google Scholar 

  23. Pap N, Beszédes S, Pongrácz E, Myllykoski L, Gábor M, Gyimes E, Hodur C, Keiski R. Microwave-assisted extraction of anthocyanins from black currant marc. Food Bioprocess Tech. 1–9 (2012)

    Google Scholar 

  24. Sutivisedsak N, Cheng HN, Willett JL, Lesch WC, Tangsrud RR, Biswas A. Microwave-assisted extraction of phenolics from bean (Phaseolus vulgaris L.). Food Res. Int. 43: 516–519 (2010)

    Article  CAS  Google Scholar 

  25. Turk MF, Baron A, Vorobiev E. Effect of pulsed electric fields treatment and mash size on extraction and composition of apple juices. J. Agr. Food Chem. 58: 9611–9616 (2010)

    Article  CAS  Google Scholar 

  26. Vito F, Ferrari G, Lebovka N, Shynkaryk N, Vorobiev E. Pulse duration and efficiency of soft cellular tissue disintegration by pulsed electric fields. Food Bioprocess Tech. 1: 307–313 (2008)

    Article  Google Scholar 

  27. Janovitz KAH, Richard FC, Goupy PM, Nicolas JJ. Kinetic studies on apple polyphenol oxidase. J. Agr. Food Chem. 38: 1437–1441 (1990)

    Article  Google Scholar 

  28. Kulshrestha S, Sastry S. Frequency and voltage effects on enhanced diffusion during moderate electric field (MEF) treatment. Innov. Food Sci. Emerg. 4: 189–194 (2003)

    Article  Google Scholar 

  29. Liu D, Vorobiev E, Savoire R, Lanoisellé JL. Intensification of polyphenols extraction from grape seeds by high voltage electrical discharges and extract concentration by dead-end ultrafiltration. Sep. Purif. Technol. 81: 134–140 (2011)

    Article  CAS  Google Scholar 

  30. Meakin PJ, Roberts JA. Dehiscence of fruit in oilseed rape (Brassica napus L.): I. Anatomy of pod dehiscence. J. Exp. Bot. 41: 995–1002 (1990)

    Article  Google Scholar 

  31. Ade-Omowaye BIO, Angersbach A, Eshtiaghi NM, Knorr D. Impact of high intensity electric field pulses on cell permeabilisation and as pre-processing step in coconut processing. Innov. Food Sci. Emerg. 1: 203–209 (2000)

    Article  CAS  Google Scholar 

  32. Pavlin M, Leben V, Miklavcic D. Electroporation in dense cell suspension— theoretical and experimental analysis of ion diffusion and cell permeabilization. Biochim. Biophys. Acta 1: 12–23 (2007)

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science, University of Otago, PO Box 56, Dunedin, 9054, New Zealand

    Sue-Siang Teh, Alaa El-Din A. Bekhit & John Birch

  2. Department of Mathematics and Statistics, University of Otago, PO Box 56, Dunedin, 9054, New Zealand

    Brian E. Niven

  3. Department of Biochemistry, University of Otago, P.O. Box 56, 710 Cumberland St, Dunedin, 9054, New Zealand

    Alan Carne

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  1. Sue-Siang Teh
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  2. Brian E. Niven
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  3. Alaa El-Din A. Bekhit
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  4. Alan Carne
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  5. John Birch
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Correspondence to John Birch.

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Teh, SS., Niven, B.E., Bekhit, A.ED.A. et al. Optimization of polyphenol extraction and antioxidant activities of extracts from defatted flax seed cake (Linum usitatissimum L.) using microwave-assisted and pulsed electric field (PEF) technologies with response surface methodology. Food Sci Biotechnol 24, 1649–1659 (2015). https://doi.org/10.1007/s10068-015-0214-9

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  • DOI: https://doi.org/10.1007/s10068-015-0214-9

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