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GintMT1 encodes a functional metallothionein in Glomus intraradices that responds to oxidative stress

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Abstract

A full-length metallothionein (MT) gene (GintMT1) was isolated from Glomus intraradices extraradical mycelium. This is the first MT gene reported in the genus Glomus, third in the Glomeromycota. Functional analysis of GintMT1 in a MT-defective Saccharomyces cerevisiae strain indicates that it encodes a functional MT. Gene expression analyses revealed that the transcript levels of GintMT1 were elevated in mycelia treated with 5 mM Cu or paraquat but inhibited in mycelia treated with 50 μM Cu or 450 μM Cd. The elevated expression of GintMT1 in the 5 mM Cu-treated mycelia together with the ability of GintMT1 to provide tolerance to a Cu-sensitive yeast suggests that GintMT1 might afford protection against Cu. Induction of GintMT1 expression by paraquat and 5 mM Cu, treatments that also produced an oxidative damage to the fungal membranes, suggests that GintMT1 may play a role in the regulation of the redox status of the extraradical mycelium of G. intraradices.

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Acknowledgements

This work was funded by the EU project Genomyca (QLK5-CT-2000-01319). Manuel González-Guerrero was supported by a FPU fellowship from the Spanish Ministry of Education, Culture and Sports. We are grateful to Dr. Maria Harrison for providing the GintMT1 partial clone and Dr. Simone Ottonello for the gift of the S. cerevisiae strain DTY113. We also want to thank Drs. José Miguel Barea and Alberto Bago for helpful discussions and Ascensión Valderas for excellent technical assistance.

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  1. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain

    M. González-Guerrero, C. Cano, C. Azcón-Aguilar & N. Ferrol

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  1. M. González-Guerrero
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  2. C. Cano
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  3. C. Azcón-Aguilar
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  4. N. Ferrol
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Correspondence to N. Ferrol.

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González-Guerrero, M., Cano, C., Azcón-Aguilar, C. et al. GintMT1 encodes a functional metallothionein in Glomus intraradices that responds to oxidative stress. Mycorrhiza 17, 327–335 (2007). https://doi.org/10.1007/s00572-007-0108-7

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