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Mangrovimonas xylaniphaga sp. nov. isolated from estuarine mangrove sediment of Matang Mangrove Forest, Malaysia

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Abstract

A Gram-staining-negative, aerobic, rod-shaped, yellow-orange-pigmented, gliding bacterium, designated as strain ST2L12T, was isolated from estuarine mangrove sediment from Matang Mangrove Forest, Perak, Malaysia. Strain ST2L12T grew at 15–39 °C, pH 6–8 and in 1–6 % (w/v) NaCl. This strain was able to degrade xylan and casein. 16S rRNA gene sequence analysis showed 95.3–92.8 % similarity to members of the genera Mangrovimonas, Meridianimaribacter, Sediminibacter, Gaetbulibacter and Hoppeia. Phylogenetic analysis indicated that it belonged to the family Flavobacteriaceae. Respiratory quinone present was menaquinone-6 (MK-6), and the DNA G+C content was 38.3 mol%. The predominant fatty acids were iso-C15:0, iso-C15:1, C15:0 and iso-C17:0 3-OH. Moreover, previous genome comparison study showed that the genome of ST2L12T is 1.4 times larger compared to its closest relative, Mangrovimonas yunxiaonensis LYYY01T. Phenotypic, fatty acid, 16S rRNA gene sequence and previous genome data indicate that strain ST2L12T represents a novel species of the genus Mangrovimonas in the family Flavobacteriaceae, for which the name Mangrovimonas xylaniphaga sp. nov. is proposed. The type strain of Mangrovimonas xylaniphaga is ST2L12T (=LMG 28914T=JCM 30880T).

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Abbreviations

H-ASWM:

High-nutrient artificial sea water medium

L-ASWM:

Low-nutrient artificial sea water medium

TLC:

Thin-layer chromatography

HPLC:

High-performance liquid chromatography

TEM:

Transmission electron microscope

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Acknowledgments

This study was supported by the Research University (RU) mangrove project Grant (1001/PCCB/870009) provided by Universiti Sains Malaysia. We thank Dr. S. Y. Foong for providing guidance during sampling and G. Priya for assisting in isolation of strain ST2L12T. G. Furusawa gratefully acknowledges the postdoctoral fellowships granted by Universiti Sains Malaysia. B. Dinesh also acknowledges the financial support provided by the MyBrain (MyMasters) scholarship supported by the Ministry of Education Malaysia (MOE).

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Authors and Affiliations

  1. Centre for Chemical Biology, Sains@USM, Universiti Sains Malaysia, No. 10, Persiaran Bukit Jambul, 11900, Bayan Lepas, Penang, Malaysia

    Balachandra Dinesh, Go Furusawa & A. A. Amirul

  2. School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    A. A. Amirul

Authors
  1. Balachandra Dinesh
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  2. Go Furusawa
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  3. A. A. Amirul
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Corresponding author

Correspondence to Go Furusawa.

Additional information

Communicated by Erko Stackebrandt.

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Dinesh, B., Furusawa, G. & Amirul, A.A. Mangrovimonas xylaniphaga sp. nov. isolated from estuarine mangrove sediment of Matang Mangrove Forest, Malaysia. Arch Microbiol 199, 63–67 (2017). https://doi.org/10.1007/s00203-016-1275-8

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  • DOI: https://doi.org/10.1007/s00203-016-1275-8

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