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Five independent loci each control monogenic resistance to gummy stem blight in melon (Cucumis melo L.)

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Abstract

Inheritance and segregation analysis demonstrated that five independent genes in melon confer monogenic resistance to foliar infection by the fungal pathogen Didymella bryoniae, resulting in the disease known as gummy stem blight (GSB). In this study, two new monogenic sources of GSB resistance were characterized. Resistance in Cucumis melo PI 482398 was monogenic dominant based on segregation analysis of F1, F2 and backcross populations, while resistance in C. melo PI 482399 showed monogenic recessive inheritance. Four accessions, PI 482398, PI 157082, PI 511890, and PI 140471, each previously known to carry monogenic dominant resistance to GSB, were intercrossed to determine genetic relationships among these resistance sources. Recovery of susceptible individuals in F2 populations confirmed that these accessions possess different resistance genes. Resistance loci were designated Gsb-1 (formerly Mc, monogenic dominant resistance from PI 140471), Gsb-2 (monogenic dominant resistance from PI 157082), Gsb-3 (monogenic dominant resistance from PI 511890), Gsb-4 (monogenic dominant resistance from PI 482398) and gsb-5 (monogenic recessive resistance from PI 482399).

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Acknowledgements

This work was funded by the Vegetable Breeding Institute, an industry consortium that supports field-based vegetable breeding at Cornell. Additional support was provided by the USDA National Plant Germplasm System and by the Vegetable and Flower Seed Permanent Research Fund of the American Seed Trade Association. J.D.F. was supported by a USDA National Needs Fellowship in Plant Biotechnology. We acknowledge the assistance of Jessica Drennan Westra and Tom Zitter with inocula and fungal cultures, Tito Zuniga for assistance with pollinations, John Jantz, Mark Henning, George Moriarty and Mary Kreitinger for technical assistance. The experiments described comply with current U.S. laws.

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  1. Department of Plant Breeding, Cornell University, Ithaca, NY 14853, USA

    J. D. Frantz & M. M. Jahn

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  1. J. D. Frantz
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  2. M. M. Jahn
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Correspondence to M. M. Jahn.

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Communicated by J. Dvorak

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Frantz, J.D., Jahn, M.M. Five independent loci each control monogenic resistance to gummy stem blight in melon (Cucumis melo L.). Theor Appl Genet 108, 1033–1038 (2004). https://doi.org/10.1007/s00122-003-1519-2

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  • DOI: https://doi.org/10.1007/s00122-003-1519-2

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