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Generation of cloned calves from different types of somatic cells

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Abstract

Six types of bovine somatic cell lines, including a granulosa cell line of Chinese red-breed yellow cattle (YGR), a granulosa cell line of Holstein cow (HGR), two skin fibroblast cell lines of two adult Holstein cows respectively (AFB1 and AFB2), a skin fibroblast cell line (FFB) and an oviduct epithelial cell line (FOV) of a Holstein fetus, were established. Somatic cell nuclear transfer (SCNT) was carried out using these cells as nuclei donor, and a total of 12 healthy calves were cloned. The effects of different types of donor cells on developmental potential of bovine SCNT embryos were investigated, (i) There was no significant difference in development rates to the blastocyst stage for SCNT embryos from YGR and HGR (33.2% and 35.1%, respectively). Pregnancy rates of them were 33.3% and 30.2%, respectively; and birth rates were 16.7% and 11.6%, respectively, (ii) Development rates to the blastocyst stage for SCNT embryos from diffetent individuals (AFB1 and AFB2) differed significantly (27.9% and 39.4%, respectively, P < 0.05). Pregnancy rates of them were 36.2% and 36.4%, respectively; and birth rates were 14.9 % and 27.3%, respectively, (iii) There was significant difference in development rates to the blastocyst stage for SCNT embryos from FFB and FOV of the same fetus (37.9% and 41.5%, respectively,P < 0.05). Pregnancy rates of them were 45.7% and 24.1%, respectively; and birth rates were 22.9 % and 10.3%, respectively. Finally, developmental potential of bovine SCNT embryos from all four types of somatic cells from Holstein cows (HGR, AFB, FFB and FOV) were compared. Forin vitro development stage, development rates to the blastocyst stage for SCNT embryos from HGR, AFB, FFB and FOV were 35.1%A, 29.4%B, 37.9%A and 41.5%C, respectively (P ABC < 0.05); forin vivo development stage, pregnancy rates of them were 30.2%, 36.2%, 45.7% and 24.1%, respectively; and birth rates of them were 11.6%, 17.2%, 22.9% and 10.3% respetively.

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References

  1. Willmut, I., Schnieke, A. E., McWhir, J. et al., Viable offspring derived from fetal and adult mammalian cells, Nature, 1997, 385: 810–813.

    Article  Google Scholar 

  2. Wakayama, T., Perry, A. C., Zuccotti, M. et al., Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei, Nature, 1998, 394: 369–374.

    Article  CAS  PubMed  Google Scholar 

  3. Kato, Y., Tani, T., Tsunoda, Y. et al., Eight calves cloned from somatic cells of single adult, Science, 1998, 282: 2095–2098.

    Article  CAS  PubMed  Google Scholar 

  4. Baquisi, A., Behboodi, E., Melican, D. T. et al., Production of goats by somatic cell nuclear transfer, Nat. Biotechnol., 1999, 17: 456–461.

    Article  Google Scholar 

  5. Wang, Y., Zou, X., Liu, J. et al., Cloned goats (Capra hircus) from foetal fibroblast cell lines, Chinese Science Bulletin, 2000, 45(1): 34–38.

    Article  Google Scholar 

  6. Polejaeva, J., Chen, S. H., Vaught, T. D. et al., Cloned pigs produced by nuclear transfer from adult somatic cells, Nature, 2000, 407: 505–509.

    Article  Google Scholar 

  7. Shin, T., Kraemer, D., Pryor, J. et al., A cat cloned by nuclear transplantation, Nature, 2002, 415: 859.

    Article  CAS  PubMed  Google Scholar 

  8. Chesne, P., Adenot, P. G., Viglietta, C. et al., Cloned rabbits produced by nuclear transfer from adult somatic cells, Nat. Biotechnol., 2002, 20(4): 366–369.

    Article  CAS  PubMed  Google Scholar 

  9. Holden, C., Genetics: First cloned mule races to finish line, Science, 2003, 300: 1354.

    Article  CAS  PubMed  Google Scholar 

  10. Galli, C., Lagutina, I., Crotti, G. et al., Pregnancy: A cloned horse born to its dam twin, Nature, 2003, 424: 635.

    Article  CAS  PubMed  Google Scholar 

  11. Zhou, Q., Renard, J. P., Le Friec, G. et al., Generation of fertile cloned rats by regulating oocyte activation, Science, 2003, 302: 1179.

    Article  CAS  PubMed  Google Scholar 

  12. Guo, J., An, Z., Li, Y. et al., Cloned goats (Capra hircus) from adult ear cells, Science in China, Ser. C., 2002, 45(3): 260–267.

    Article  CAS  Google Scholar 

  13. Chen, D., Li, J., Han, Z. et al., Somatic cell bovine cloning: Effect of donor cell and recipients, Chinese Science Bulletin, 2003, 48(6): 549–554.

    Article  CAS  Google Scholar 

  14. Gong, G., Dai, Y., Fan, B. et al., Production of transgenic blastocysts by nuclear transfer from different types of somatic cells in cattle, Science in China, Ser. C, 2004, 47(2): 183–189.

    Article  CAS  Google Scholar 

  15. Zimmermann, U., Vienken, J., Electric field-induced cell-to-cell fusion, J. Membrane Biol., 67: 165–182.

  16. Wilmut, I., Beaujean, N., de Sousa, P. A. et al., Somatic cell nuclear transfer, Nature, 2002, 419: 583–586.

    Article  CAS  PubMed  Google Scholar 

  17. Hill, J. R., Burghardt, R. C., Jones, K. et al., Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses, Biol. Reprod., 2000, 63: 1787–1794.

    Article  CAS  PubMed  Google Scholar 

  18. Rideout, W. M., Eggan, K. and Jaenisch, R., Nuclear cloning and epigenetic reprogramming of the genome, Science, 2001, 293: 1093–1098.

    Article  CAS  PubMed  Google Scholar 

  19. Garry, F. B., Adams, R., McCann, J. P. et al., Postnatal characteristic of claves produced by nuclear transfer cloning, Theriogenology, 1996, 45: 141–152.

    Article  Google Scholar 

  20. Kang, Y. K., Koo, K. B., Park, J. S. et al., Aberrant methylation of donor genome in cloned bovine embryos, Nat. Genet., 2001, 28: 173–177.

    Article  CAS  PubMed  Google Scholar 

  21. Xue, F., Tian, X. C., Du, F. et al., Aberrant patterns of X chromosome inactivation in bovine clones, Nat. Genet., 2002, 31: 216–220.

    Article  CAS  PubMed  Google Scholar 

  22. Wells, D. N., Misica, P. M., Tervit, H. R., Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells, Biol. Reprod, 1999, 60: 996–1005.

    Article  CAS  PubMed  Google Scholar 

  23. Kubota, C., Yamakachi, H., Yang, X. et al., Six cloned calves produced from adult fibroblast cells after long-term culture, Proc. Natl. Acad. Sci. USA, 2000, 97: 990–995.

    Article  CAS  PubMed  Google Scholar 

  24. Cibelli, J. B., Stice, S. L., Golueke, P. J. et al., Cloned transgenic calves produced from nonquiescent fetal fibroblasts, Science, 1998, 289: 1256–1258.

    Article  Google Scholar 

  25. Zakhartchenko, V., Durcova-Hills, G., Stojkovic, M. et al., Effects of serum starvation and re-cloning on the efficiency of nuclear transfer using bovine fetal fibroblasts, J. Reprod. Fertil., 1999, 115 (2): 325–331.

    CAS  PubMed  Google Scholar 

  26. Gong, G., Dai, Y., Fan, B. et al., Production of transgenic claves by somatic cell nuclear transfer, Chinese Science Bulletin, 2004, 49(2): 161–166.

    Article  CAS  Google Scholar 

  27. Kato, Y., Yabuuchi, A., Motosugi, N. et al., Developmental potential of mouse follicular epithelial cells and cumulus cells after nuclear transfer, Biol. Reprod., 1999, 61: 1110–1114.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, 100094, Beijing, China

    Guochun Gong, Yunping Dai, Haiping Wang, Lili Wang, Rong Li, Rong Wan, Ying Liu & Ning Li

  2. Institute of Animal Science, Chinese Academy of Agricultural Science, 100094, Beijing, China

    Huabing Zhu

Authors
  1. Guochun Gong
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  2. Yunping Dai
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  3. Huabing Zhu
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  4. Haiping Wang
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  5. Lili Wang
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  6. Rong Li
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  7. Rong Wan
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  8. Ying Liu
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  9. Ning Li
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Correspondence to Ning Li.

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These authors contribute equally to this work.

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Gong, G., Dai, Y., Zhu, H. et al. Generation of cloned calves from different types of somatic cells. Sci. China Ser. C.-Life Sci. 47, 470–476 (2004). https://doi.org/10.1007/BF03187106

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  • DOI: https://doi.org/10.1007/BF03187106

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