Abstract
The possibility of using a combination of the mutation process with the induction of the repair processes has been studied to increase the mutation frequencies in algal populations after UV-treatment. From this study it follows that the repair process induced by visible light is much more effective than the dark repair processes in the chlorococcal algae used. In these algae, visible light perhaps does not induce only those repair processes which affect their DNA, but probably also some recovery ones which affect their damaged structures and physiological functions. A suitable combination of the sensitization of algal cells by a DNA-base analogue before UV-treatment and the induction of the light repair and recovery processes resulted in a rather high increase of viable mutations in chlorococcal algae. These findings may be useful in the breeding of chlorococcal algae, which have no possibility of hybridization (except somatic).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baalen van, Ch.: The effects of ultraviolet irradiation on a coccoid blue-green alga: survival, photosynthesis and photoreactivation. - Plant Physiol.43: 1689–1695, 1968.
Baalen van, Ch., O’Donnell, R.: Action spectra for ultraviolet killing and photoreactivation in blue-green algaAgmenellum quadruplicatum. - Photochem. Photobiol.15: 269–274, 1972.
Bridges, B. A., Munson, R. J.: UV-mutagenesis. - Stud. biophys.19: 49–57, 1969.
Davies, D. R.: The control of dark repair mechanisms in meiotic cells. - Molec. gen. Genet.100: 140–149, 1967a.
Davies, D. R.: UV-sensitive mutants ofChlamydomonas reinhardtii. - Mutation Res.4: 756 to 770, 1967b.
Davies, D. R., Levin, S.: UV-induced reversion to prototrophy in three strains ofChlamydomonas reinhardtii differing in dark repair capacity. - Mutation Res.5: 231–236, 1968.
FabRe, F.: A UV-supersensitive mutant in the yeastSchizosaccharomyces pombe. - Molec. gen. Genet.110: 134–143, 1971.
Giese, A. C.: Ultraviolet action spectra in perspective with special reference to mutation. - Photochem. Photobiol.8: 527–546, 1968.
Guialis, A., DeeRing, R. A.: Repair of deoxyribonucleic acid in ultraviolet light-sensitive and -resistantDictyoslelium discoideum strains. - J. Bacteriol.127: 59–66, 1976.
Halldal, P., TaUbe, Ö.: Ultraviolet action and photoreactivation in algae. - In:Giese, A. C. (eds) Photophysiology. Vol. 7. Pp. 163–185. Acad. Press, New York-London 1972.
Kemp, C. L.: Liquid-holding and recovery from UV-induced damage inEudorina elegans. - Arch. Mikrobiol.86: 255–264, 1972.
Kemp, C. L., MaLLaoy, K. M.: 4-nitroquinoline-l-oxide induced lethality and repair in the algaEudorina elegans. - Mutation Res.20: 191–200, 1973.
Kemp, C. L., Malloy, K. M.: The influence of acridine dyes and caffeine on recovery from ultraviolet damage inEudorina elegans. - Can. J. Microbiol.21: 1849–1854, 1975.
Kemp, C. L., Tsao, M. S., Thorson, G.: Ultraviolet light induced thymine dimers and repair processes in the algaEudorina elegans. - Can. J. Microbiol.18: 1809–1815, 1972.
Kemp, C. L., Wentworth, J. W.: Ultraviolet radiation studies on the colonial algaEudorina elegans. - Can. J. Microbiol.17: 1417–1424, 1971.
Kuzin, A. M.: Evolution of our knowledge of the role of primary and initial processes in the formation of radiobiological effects.- Stud. biophys.53: 1–4, 1975.
Li, S. L., Rédei, G. P., Gowans, C. S.: Phylogenetic comparison of mutation spectra. -Molec. gen. Genet.100: 77–83, 1967.
Nečas, J.: Influence of light on the autospore number of some chlorococcal algae. - Biol. Plant.11: 465–469, 1969.
Nečas, J.: Is methyl ethanesulphonate a suitable mutagen for chlorococeal algae? - Arch. Hydrobiol. (Suppl. 39) Algol. Stud.4: 226–238, 1971.
Nečas, J.: Attempts to sensitize some chlorococcal algae using 5-bromouracil for the induction of mutations by UV-light. -Biochem. Physiol. Pflanzen166: 115–127, 1974.
Nečas, J.: Physiological and mutagenic effects of N-methyl-N’-nitro-N-nitrosoguanidine in populations of chlorococcal algae. - Biol. Plant.17: 130–138, 1975.
Nečas, J.: Sensitization of three strains of chlorococcal algae for UV-effects by 5-bromodeoxy-uridine. - Biol. Plant.18: 1–12, 1976a.
Nečas, J.: Sensitization dependence of UV-irradiation effects on the concentration of 5-bromo-deoxyuridine in a precultivation medium for a chlorococcal alga. - Biochem. Physiol. Pflanzen170: 487–500, 1976b.
Nečas, J.: [Mutations in algal populations and their manifestation on cell colonies growing on the surface of a solid medium.] In Czech. -Biol. Listy (Praha)41: in press, 1977.
Parker, K. E., Horsley, R. J.: The ultraviolet radiosensitivity ofOedogonium cardiacum cells at various stages of the cell cycle. - Radiat. Bot.12: 239–248, 1972.
Rosen, H., Ebersold, W. T.: Recombination in relation to ultraviolet sensitivity inChlamydomonas reinhardtii.- Genetics71: 247–253, 1972.
Šetlík, I., Berková, E., Doucha, J., Kubín, Š., VendlovÁ, J., Zachleder, V.: The coupling of synthetic and reproduction processes inScenedesmus quadricauda. - Arch. Hydrobiol. (Suppl. 41) Algol. Stud.7: 172–213, 1972.
Sulek, J.: Nuclear division as a criterion of synchrony in cultures ofScenedesmus quadricauda (Turp.)Bréb. -Arch. Hydrobiol. (Suppl. 41) Algol. Stud.6: 72–93, 1972.
Sulek, J.: Nuclear division inScenedesmus quadricauda (Turp.)Bréb - Arch. Hydrobiol. (Suppl. 46) Algol. Stud.12: 224–258, 1975.
Taube, Ö.: Ultraviolet inactivation and photoreactivation in synchronizedChlorella. - Physiol. Plant.33: 755–761, 1970.
Witkin, E. M.: Ultraviolet-induced mutation and DNA repair. - Annu. Rev. Genet.3: 525 to 552, 1969.
Witkin, E. M.: Persistence and decay of thermoinducible error-prone repair activity on nonfilamentous derivatives of tif-1Escherichia coli B/r: The timing of some critical events in ultraviolet mutagenesis. - Molec. gen. Genet.142: 87–103, 1975.
Zhevner, V. D., Shestakov, S. V.: Studies on the ultraviolet sensitive mutants of blue-green algaSynechocystis aquatilis Sanv. - Arch. Microbiol.86: 349–360, 1972.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nečas, J. Combination of the mutation process with the sensitization and repair processes leading to increased frequencies of mutations in algal populations. Biol Plant 19, 173–182 (1977). https://doi.org/10.1007/BF02923106
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02923106